Long-acting Cytokine Expressing Humanized Mouse Models for CART In Vivo Testing

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Long-acting Cytokine Expressing Humanized Mouse Models Is Important for CART In Vivo Testing

Humanized mouse models play an important role in evaluating the drug safety and efficacy of immunotherapy. They have been widely employed due to their ease of use and good reproducibility. However, difficulties such as imperfect reconstruction of the human immune system hinder the development of humanized mouse models. Evidence suggests that expressing cytokines in humanized mice can help reconstruct humanized mouse models with fully human immune systems. Therefore, there is an urgent need to develop long-acting cytokine-expressing humanized mouse models.

Fig. 1 The application of humanized mouse models in CART.¹

Our Long-acting Cytokine Expressing Humanized Mouse Models for CART In Vivo Testing

To solve these obstacles, Creative Biolabs developed long-acting cytokine-expressing humanized mouse models for CART in vivo testing. We take advantage of long-acting cytokine delivery technology to construct humanized mouse models with complete immune systems, which promisingly enhance the ratio of myeloid cells and lymphoid cells in humanized mice. In this service, we provide a wide range of well-developed long-acting cytokine-expressing humanized mouse models for customers' immediate application. In addition, we provide several delivery strategies to deliver the target gene into the humanized mice. For the application of long-acting cytokine-expressing humanized mouse models into the evaluation of CART, we also offer some associated services, such as CART engineering, for your convenience in efficiently accomplishing your projects. At Creative Biolabs, we are confident in delivering the desirable outcomes for every client in a short turnaround.

Fig. 2 Vector development and in vivo CAR therapy.²

Highlights of Our Long-acting Cytokine Expressing Humanized Mouse Models

Long-acting cytokine expressing;
Ready to use;
Longer efficacy window.

Delivery Strategies

We provide three common delivery strategies to help you introduce the desirable targets into humanized mice to develop appropriate humanized mouse models:

Hydrodynamic Injection
Pronuclear Injection
Knock-In

Humanized Mouse Strains

Additionally, we provide several humanized mouse strains to choose from for the construction of humanized mouse models:

Fig. 4 Humanized mouse strains. (Creative Biolabs Original)

Published Data

DATA: The objective of this research endeavor was to examine the viability of utilizing anti-CD19 CAR-T cell therapy for the treatment of SLE in a murine disease model. By injecting the matching CAR-T cells into MRL-lpr mice, assessed the therapeutic efficacy of murine anti-CD19 CARs with CD28 or 4-1BB intracellular costimulatory motif. In addition, before the onset of the disease, anti-CD19 CAR-T cells were introduced into MRL-lpr mice to ascertain their potential function in the prevention of SLE. The results indicated that anti-CD19 CAR-T cell adoptive transfer resulted in a greater long-lasting reduction of B-cells in MRL-lpr mice compared to antibody therapy. In addition to preventing disease development, the transplantation of syngeneic anti-CD19 CAR-T cells also demonstrated therapeutic effects later in the course of the illness.

Fig. 3 The effectiveness of CAR-T cells in the mouse model of SLE.³

For more details about our long-acting cytokine-expressing humanized mouse models for CART in vivo testing, please feel free to get in touch with us.

References

Yue, Hanwei, and Lin Bai. "Progress, implications, and challenges in using humanized immune system mice in CAR‐T therapy—Application evaluation and improvement." Animal Models and Experimental Medicine (2023).
Michels, Alexander, et al. "Precision medicine: In vivo CAR therapy as a showcase for receptor-targeted vector platforms." Molecular Therapy (2022).
Jin, Xuexiao, et al. "Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus." Cellular & molecular immunology 18.8 (2021): 1896-1903.