Multicolor Fluorescent Spot Assay Service

Online Inquiry

Creative Biolabs offers MFSA service to help you measure multiple cytokines and immune cell types to obtain quantitative data for each target and gain insight into the dynamics and balance of the immune response.

MFSA: A Powerful Technique in Cytokine Detection

The Multicolor Fluorescent Spot Assay (MFSA) is a powerful technique used in immunology and cell biology to simultaneously detect and quantify multiple analytes or cell types in a single sample. This assay leverages the specificity of antibodies and the sensitivity of fluorescent dyes to provide detailed information about the presence and quantity of various targets within a sample.

Fig.1 Triple cytokine assay. (Janetzki, Sylvia et al., 2014) Fig.1 Image of triple cytokine assay.¹

MFSA at Creative Biolabs

MFSA is a novel detection method based on enzyme-linked immunospot spot (ELISpot). It enables simultaneous measurement of the secretion of different cytokine combinations at a single CAR-T level.

Detection Process of MFSA
The following is a workflow for the detection of multiple cytokines in a sample using MFSA method:

By following these steps, MFSA can provide detailed and quantitative data for multiple targets in a single sample, combining the numerical evaluation of CAR-T products with their functional characterization.

Advantages of MFSA
As a powerful analysis tool, we've listed some of the advantages of the MFSA approach:
- Multiple cytokines can be detected simultaneously, increasing throughput and efficiency.
- The use of specific antibodies and sensitive fluorescent dyes ensures accurate detection.
- Quantitative data on the abundance of each target in the sample can be provided.
- MFSA is suitable for a wide range of sample types, including cells, tissues, and biofluids.

Applications of MFSA

In addition to measuring the secretion levels of multiple cytokines in CAR-T samples, MFSA can also be used for other purposes:

Multicolor Fluorescent Spot Assay is a valuable tool in research and clinical diagnostics, offering detailed insights into complex biological systems through its ability to analyze multiple parameters simultaneously. If you need simultaneous detection of multiple cytokines in the CART field, you can contact us for the corresponding service.

Reference

Janetzki, Sylvia et al. "Stepping up ELISpot: multi-level analysis in FluoroSpot assays." Cells. (2014) 3,4 1102-15.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Key Updates

NEWSLETTER

The latest newsletter to introduce the latest breaking information, our site updates, field and other scientific news, important events, and insights from industry leaders

LEARN MORE NEWSLETTER

NEW SOLUTION

CellRapeutics™ In Vivo Cell Engineering: One-stop in vivo T/B/NK cell and macrophage engineering services covering vectors construction to function verification.

LEARN MORE SOLUTION

Novel Solution. (Creative Biolabs Authorized)

NOVEL TECHNOLOGY

Silence™ CAR-T Cell: A novel platform to enhance CAR-T cell immunotherapy by combining RNAi technology to suppress genes that may impede CAR functionality.

LEARN MORE NOVEL TECHNOLOGY

New Technology. (Creative Biolabs Authorized)

NEW SOLUTION

Canine CAR-T Therapy Development: From early target discovery, CAR design and construction, cell culture, and transfection, to in vitro and in vivo function validation.

LEARN MORE SOLUTION