pCDCAR1 TAG-72 h(b2cζ), T(CAR-T-2-M325-CZ)
The vector of anti-TAG-72 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human TAG-72. The T cell is genetically modified through transduction with a lentiviral vector expressing scFv of TAG-72 antibody linked to b2c and CD3-zeta signaling domains. And the vector product was designed for the treatment of colon cancer.
Targeting Cell Type
Discription of Signaling Cassetes
The b2c protein belongs to high-sulphur keratin family which comprises at least three proteins, B2A, B2B and B2C. And b2c in chimeric antigen receptors improve T-cell effector functions and enhance anti-tumor activity.
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Tumor-associated glycoprotein 72
TAG-72; mucin antigen; Tumor-associated glycoprotein; Colon cancer; scFv; FcεRIγ; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; CC49
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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