Personalized Humanized Mouse Models for CART In Vivo Testing

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The Urgency of Constructing Personalized Humanized Mouse Models

Humanized mouse models serve as a valuable tool for the development of cancer therapy. They offer a more dependable comprehension of intricate therapeutic interventions. Nowadays, the humanized mouse model has evolved to have a more complete human immune system, which delivers more convenience in cancer therapy. However, mismatched tumors and immune systems hinder the application of these humanized mouse models in evaluating most immunotherapies. Therefore, there is an urgent need to develop personalized humanized mouse models to evaluate different drugs and immunotherapies.

Fig. 1 Precise Treg-based cancer immunotherapies are developed using HIS mouse models.¹

Our Personalized Humanized Mouse Models for CART In Vivo Testing

Creative Biolabs specializes in providing personalized humanized mouse models for CART in vivo testing. We provide numerous types of specially personalized humanized mouse models targeting diverse therapeutic intents for customers to meet your diverse needs. Simultaneously, we deliver a set of complete experiments to examine animal models for global customers. Furthermore, we are confident in providing customized development solutions if customers have specific target needs. At Creative Biolabs, we have great confidence in presenting an ideal personalized humanized mouse model to serve as a useful tool in achieving your projects.

Fig. 2 Models of peritoneal carcinomatosis in mice.²

Successful Case

Here we list some successful cases in personalized humanized mouse models:

A personalized mouse model with tumor-like tumor-specific immune systems is developed using bone marrow cells harvested from tumor patients or peripheral blood leukocytes stimulated by G-CSF.
A humanized mouse model capable of autologous HLA-dependent differentiation and maturation of human T cells, which was achieved by the transplantation of autologous thymic epithelial cells.
A humanized mouse model was constructed by using the patient's iPS cells to differentiate thymic epithelial cells in vitro, which is useful to replace the patient's thymus tissue thereby promoting the maturation of immune system T cells.
A murine FCRR knockout humanized mouse model is used to evaluate antibody drugs.

Highlights

Personalized: We provide personalized products and services to present the desirable humanized mouse models for every client.
Highly effective: Our personalized humanized mouse models will be a great useful tool to give a rapid reaction to your stimulus.
Reliable quality: We implement strict quality control to ensure the excellent quality endowed with our products and services.
After-sale warranty: Our experienced research team will solve every related problem to aid in your projects running smoothly.

Personalized Humanized Mouse Models

We provide several well-developed ready-to-use personalized humanized mouse models for customers to support your diverse projects:

Models	Modification	Application
NSG hIL2-high	High expression of IL-2.	Used to validate Tils, TCR-T, and solid tumor CAR-T.
NSG hIL2-low	Lowly expression of IL-2.	Tregs reconstitution in vivo.
NSG GM3	Overexpression of GM-CSF and IL3.	Myeloid cell reconstruction; AML (PDX), CAR-Mac in vivo growth.
NSG hIL6	Endogenously expression of IL-6.	Macrophage development; MM(PDX) in vivo growth.
NSG hIL15-high	High expression of IL-15.	Reconstitution of ADCC, CAR-NK, and iPS-CAR-NK in vivo
NSG hIL15-low	Lowly expression of IL-15.	Reconstitution of ADCC and CD34-NK.
NSG HLA A2	Fusion expression of human B2m and HLA A2.	The growth and maturation of human T cells.
NSG FLT3 KO / FLT3L	Knockout of endogenous FLT3; Humanized of FLT3LG.	The development of the myeloid and dendritic cells.
NSG hCD47	Humanization of CD47.	Inhibition of anemia associated with human myeloid to prolong their lifespan.
NSG FcyR KO	Knockout of murine FcyRI, Il, Ill, and IV.	Knockout of endogenous ADCP; Enhance the reliability of antibody studies.
NSG MHC-I KO	Knockout of mouse H-2Db and H-2Kd.	Reduction of the GVHD in PBMC mouse.
NSG mTslp	Overexpression of mouse Tslp.	The development of lymph nodes.
NSG LTi (IL2Ry)	Expression of IL2Ry in LTi cells.	The development of lymph nodes.
NSG hHER2	Humanization of HER2.	Off-target cytotoxicity of HER2-targeted drugs (CAR-TADC).
HSC NSG Plus3	Triple expression of IL3, GMCSF, and IL15.	Reconstitution of the complete immune system (TB, NK, myeloid).
BRG hCD40	Humanization of CD40 in BRG.	Examination of CAR-T cytokine storm.
hCD3 EDG	Humanization of CD3(EDG) gene cell surface region.	Bispecific antibodies targeting CD3.
hFcyR	In situ humanization of the mouse FcvR locus.	Research and development of therapeutic antibodies.
hFcRn / HSA	Humanization of FcRn and serum albumin.	In vivo metabolism assay of antibody- and HSA-related long-acting drugs.

If you want to know more about our personalized humanized mouse models for CART in vivo testing, please contact us.

References

Serr, Isabelle, et al. "Advances in human immune system mouse models for personalized treg-based immunotherapies." Frontiers in Immunology 12 (2021): 643544.
Bella, Ángela, et al. "Mouse models of peritoneal carcinomatosis to develop clinical applications." Cancers 13.5 (2021): 963.