CellRapeutics™ Synthetic Cytokine Receptor Construction Service

Synthetic Cytokine Receptor

Prolonged stimulation leads to functional exhaustion and decreased efficacy of the CAR-T cells. To address this issue, researchers have developed a synthetic cytokine receptor that can be incorporated into CAR-T cells to control their activation. A synthetic cytokine receptor is a genetically engineered protein that has been designed to mimic the function of natural cytokine receptors. It specifically recognizes and binds to an artificial ligand to precisely control the activation of CAR-T cells in a customizable and tunable manner.

Design and Construction of Synthetic Cytokine Receptor at Creative Biolabs

A common design for a synthetic cytokine receptor contains the fusion of the extracellular domain of an artificial ligand, such as the VHHs, with the intracellular signaling domain of the cytokine receptor. Creative Biolabs offers synthetic cytokine receptor construction service, thus allowing for improvement of the safety and efficacy of CAR-T cell therapy. Our experts design synthetic cytokine receptor systems that can specifically respond to non-physiological ligands (such as fluorescent proteins) without interference from endogenous cytokines or other molecules in the biological system. This system is designed to eliminate false signals and provide a controllable and precise response to artificial ligands, allowing for more targeted and reliable cellular signaling. We utilize soluble VHHs as the ligand-binding domain to fine-tune the signaling pathways and cellular responses triggered upon ligand binding.

Workflow

We offer comprehensive design and construction services for synthetic cytokine receptors.

• Ligand Binding Domain Design: Select suitable non-physiological ligands and design a ligand-binding domain that will specifically recognize the target ligand.
• Receptor Selection: Determine the structure of intracellular signaling domains of the cytokine receptor according to your requirements.
• Cloning: Clone the genes encoding the synthetic cytokine receptor and any associated signaling components into a suitable expression vector.

What We Can Offer

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Key Advantages

• Customized Receptor Design & Construction: Rational design of chimeric receptors tailored to specific cytokine axes (e.g., IL-2, IL-7, IL-15) and precise expression requirements in your target T-cell population.
• One-Stop Functional Enhancement: Complete end-to-end development, from initial receptor sequence design and optimization to final functional in vitro T-cell validation, ensuring your cells are engineered for success.
• Engineered Persistence & Efficacy: Development of T-cells with superior proliferation and long-term persistence capabilities, effectively counteracting the inhibitory, low-cytokine environment of solid tumors.

FAQ

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Related Services

More about Our Services

Creative Biolabs is dedicated to next-generation CAR-T cell development services. Our team of experts specializes in the development, optimization, and customization of synthetic receptors to meet the specific needs of our clients. We also transfer the gene of synthetic cytokine receptors into CAR-T cells and validate relevant biological functions. Our experienced team collaborates closely with our clients to understand their project requirements, optimize the design parameters, and deliver high-quality solutions that exceed expectations. If you are considering developing a synthetic cytokine receptor, please contact us to discuss your project requirements and explore how we assist you in constructing a synthetic cytokine receptor for your specific application.

Reference

1. Engelowski, Erika, et al. "Synthetic cytokine receptors transmit biological signals using artificial ligands." Nature communications 9.1 (2018): 2034.