VivoCAR™ Anti-CD276 (C4) h(CD28-41BB-CD3z) CAR saRNA (XS-0326-XU19)

Name: VivoCAR™ Anti-CD276 (C4) h(CD28-41BB-CD3z) CAR saRNA
SKU: XS-0326-XU19
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-CD276 (C4) h(CD28-41BB-CD3ζ) CAR saRNA utilizes a self-amplifying RNA (saRNA) system to drive the expression of the C4 single-domain antibody. In the context of in vivo CAR-T reprogramming, the C4 clone is valued for its high-affinity binding to the immunoglobulin variable (IgV)-like domain of CD276 (B7-H3), a feature critical for counteracting tumor-mediated immunosuppression. The saRNA platform enables a low initial dose to achieve sustained CAR expression via intracellular RNA amplification, positioning this approach as an ideal strategy for efficient, dose-sparing modification of T cells directly within the patient.

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CAR Information

Target
CD276

Target Species
Human

Antibody Clone
C4

scFv Host Species
Lama pacos

Receptor Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation CD28-41BB-CD3ζ architecture employs a sequential costimulatory strategy, where the CD28 module drives rapid initial activation and metabolic reprogramming, while the 4-1BB component sustains long-term cellular viability and mitigates exhaustion. This tandem design, anchored by the CD3ζ signaling chain, synergistically amplifies downstream signal transduction, resulting in a balanced T-cell phenotype with robust effector capacity and prolonged durability. The integrated signaling cascade thus optimizes both kinetic expansion and persistence, enhancing antitumor efficacy.

CAR saRNA Properties

Structure
Linear

Cap
Cap 1

PolyA
Segmented PolyA

Product Type
CAR IVT saRNA

Concentration
Lot specific

Buffer
1 mM Sodium Citrate, pH 6.4 (or specialized RNA storage buffer, RNase-free).

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

saRNA Quality Control

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Target Information

Full Name
B7-H3 immune checkpoint molecule

Alternative Names
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; B7 homolog 3; B7-H3 antigen

Introduction
CD276 is a member of the B7 family of immune checkpoint proteins. It is overexpressed in various solid tumors and plays a role in regulating the immune response and promoting tumor invasion.

Targeting Diseases
Prostate Cancer, Neuroblastoma, Melanoma, Renal Cell Carcinoma, Lung Cancer, Ovarian Cancer

UniProt ID
Q5ZPR3

Gene ID
80381

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.