VivoCAR™ Anti-HMW-MAA (LC007) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX100)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-HMW-MAA (LC007) h(CD28-41BB-CD3z) CAR circRNA leverages the LC007 clone, specifically selected for its high specificity toward the core protein of HMW-MAA in triple-negative breast cancer (TNBC). In the realm of in vivo engineering, this clone is vital for identifying aggressive, poorly differentiated malignant cells. The circular RNA architecture provides a durable expression profile on host T cells, allowing for sustained surveillance in the metastatic niche. This safe, repeatable strategy targets the underlying oncogenic scaffold of solid tumors while ensuring the payload is cleared before causing chronic off-tumor effects.

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Target Information

  • Target
  • HMW-MAA
  • Common Name
  • HMW-MAA,CSPG4
  • Alternative Names
  • NG2, MCSP, MCSPG, MSK16, CSPG4A, CSPG4, MEL-CSPG
  • Full Name
  • High Molecular Weight Melanoma-Associated Antigen
  • Introduction
  • HMW-MAA is a large membrane-bound chondroitin sulfate proteoglycan. It is highly and universally expressed on the surface of most Melanoma cells (over 90%), as well as cells from neuroectodermal origin, but is largely restricted in expression on normal adult tissues. Its consistent and high expression on the cell surface makes it a historically and continuously relevant target for immunotherapy against melanoma, which is typically a difficult-to-treat solid tumor.
  • Targeting Diseases
  • Melanoma, Neuroblastoma, Certain Sarcomas (Chondrosarcoma)
  • Target Species
  • Human
  • Antibody Clone
  • LC007
  • scFv-Host Species
  • Mouse
  • Receptor_Construction
  • CD28-41BB-CD3z
  • Generation
  • Third
  • Description of Signaling Cassetes
  • The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.
  • Structure
  • Circula
  • Product Type
  • CAR IVT circRNA
  • Concentration
  • Lot specific
  • Storage
  • Aliquot and store at -80°C. Avoid freeze/thaw cycles.
  • Handling
  • Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.
  • IVT Vector Sequencing
  • Pass
  • IVT Vector Enzyme Digestion
  • Pass
  • Purity OD260/280
  • Pass
  • RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
  • Pass
  • RNA Sequence Assessment
  • Pass
  • Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
  • Pass
  • Endotoxin (<10 EU/mg)
  • Pass
  • In Vitro Transfection and Expression (Optional)
  • Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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