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EXPLORE MORE HighlightsAll products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
TCR J15 is specific for H60, a dominant minor histocompatibility antigen. J15 TCR transgenic T cells express transgenic TCR β chains containing variable segments composed of Vβ8.3 (Vβ 13-1-02 according to the nomenclature defined in the international immunogenetics information system). Although expression of the transgenic TCR α chain could not be confirmed directly due to the lack of a suitable antibody to stain the transgenic Vα13D-1, staining with H60-tetamer confirmed expression of transgenic TCR by recognizing the H60 peptide/H-2Kb complex. Furthermore, negative selection of thymocytes expressing the J15 TCR in the thymus where H60 is ubiquitously expressed verified the specificity of the J15 TCRs.
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