pCDCAR1 HIV-1 gp120 h(28ζ)(CAR-LC291)

The vector of anti-HIV-1 chimeric antigen receptor (CAR) is a ligand-based CAR constructed for the engineering of T cells to target HIV-1. The T cells are genetically modified through transduction with a lentiviral vector expressing CD4 and scFv of anti-HIV-1 gp120 antibody linked to CD28 and CD3ζ signaling domains through a 10aa linker. The scFv moiety prevents the CD4 moiety of the CAR from functioning as an HIV entry receptor. And the vector product was designed for the treatment of HIV infection.


Details


Target
HIV-1 gp120
Target Species
Human
Targeting Cell Type
T cell
Targeting Diseases
HIV infection
Generation
Second
Vector Name
pCDCAR1
Vector Length
~8kb
Vector Type
Retroviral
Receptor Construction
CD4-10-scFv-CD28-CD3ζ
Discription of Signaling Cassetes
CD28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Antibody Clone
17b
Antibody Host
Human

Target


Gene Name
HIV-1 envelope glycoprotein gp120
Official Symbol
HIV-1 gp120
Gene ID
Synonyms
HIV-1 gp120; HIV-1 envelope glycoprotein gp120

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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CONTACT US

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
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Tel: 44-207-097-1828

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