CD24

Product List

Based on the continuous development of CAR-T therapies with CD24-targeting antibodies, Creative Biolabs has developed a comprehensive series of CD24 molecule-related products through the design and construction of CAR, including CAR vector products, CAR BiTE products, CAR cell products, CAR mRNA, etc. You can filter the keywords below to find the products you need.

Background

CD24, as a cell adhesion molecule, is a protein encoded by the human CD24 gene. It is also known as a cluster of differentiation 24 or thermostable antigen CD24. Studies have shown that CD24 is expressed on the surface of B lymphocytes, differentiated neuroblasts, neutrophils, and neutrophil precursors. Functional studies have shown that CD24 contributes to downstream signaling networks as well as neurodevelopment. In addition, it induces apoptosis by cross-linking on the surface of neutrophils.

Associated Diseases

• Ovarian cancer
• Erythroleukemia
• Multiple Sclerosis
• Rheumatoid Arthritis
• Systemic Lupus Erythematosus

Anti-CD24 CAR-T Services

Anti-CD24 CAR-T In Vitro Assays

Anti-CD24 CAR-T Expression Test

With appropriate assays, CD24 expression levels in different cell lines can be quantified. Creative Biolabs offers different CAR expression assays, including flow cytometry, western blotting, and CAR-specific monoclonal antibody development, to help you achieve chimeric antigen receptor (CAR) expression and stability testing.

Fig.1 Analysis of CD24 antigen expression in different ovarian cancer cell lines using flow cytometric. The results showed that the CD24 expression of SKOV3 and OVCAR3 cells was relatively higher than that of A2780 and 293T cells.¹

Anti-CD24 CAR-T Cytokine Release Test

ELISA can quantify CD24-CAR-NK-92 cell activation to measure IFNγ secretion levels. Based on the CellRapeutics™ platform, Creative Biolabs offers a range of cytokine enzyme-linked immunosorbent assay (ELISA) kits for the detection of different cytokine secretions.

Fig.2 IFNγ levels of anti-CD24-CAR-NK-92 cells co-incubated with CD24-expressing cancer cells were determined by ELISA.¹

Anti-CD24 CAR-T In Vitro Cytotoxicity Assay

Studies have shown that CD24-specific NK cells have a high killing efficiency against OVCAR3 and SKOV3 cells. Creative Biolabs uses cytotoxicity analysis to assess the toxicity of CAR-transplanted T cells or NK cells to normal cells. In addition, we can also co-express CARs with appropriate suicide genes to kill cancer cells without affecting normal cells.

Fig.3 Cytotoxicity of anti-CD24-CAR-NK-92 cells. The results showed that anti-CD24-CAR conferred the ability of NK-92 cells to specifically kill antigen-presenting cells.¹

Anti-CD24 CAR-T In Vivo Assays

Leveraging our proprietary CellRapeutics™ platform, Creative Biolabs provides one-stop, customized CD24 CAR-T in vivo testing services to customers worldwide. Our CD24 CAR-T in vivo assays cover a full range of high-quality services, from animal model construction to in vivo efficacy and toxicity evaluation. You can find more information about what you are interested in the CAR-T Preclinical In Vivo Testing unit.

Reference

1. Klapdor, Rüdiger.; et al. "Characterization of a novel third-generation anti-CD24-CAR against ovarian cancer." International journal of molecular sciences. (2019). 20,3 660.