VivoCAR™ Anti-EPCAM (7-F17) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX32)

Name: VivoCAR™ Anti-EPCAM (7-F17) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX32
Rating: 5 (1 reviews)

Online Inquiry Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-EPCAM (7-F17) h(CD28-41BB-CD3z) CAR circRNA features the 7-F17 clone, optimized for robust binding to high-density EPCAM clusters within primary tumor masses. For in vivo CAR-T applications, the 7-F17 clone is vital for maintaining sustained immunological synapses in the presence of competing adhesion molecules. Combined with the circRNA platform's high translation efficiency, this product provides a concentrated therapeutic pulse to target epithelial solid tumors, offering a sophisticated non-viral tool that eliminates the risk of insertional mutagenesis.

Add to Cart

Target Information

Target
EPCAM

Common Name
EPCAM

Alternative Names
ESA, KSA, M4S1, MK-1, DIAR5, EGP-2, EGP40, KS1/4, MIC18, TROP1, BerEp4, EGP314, HNPCC8, LYNCH8, MOC-31, Ber-Ep4, TACSTD1

Full Name
Epithelial Cell Adhesion Molecule

Introduction
EpCAM is a pan-carcinoma antigen highly expressed on the surface of most epithelial-derived cancers (carcinomas), including colorectal, gastric, breast, and ovarian cancers. Its limited expression on basal stem cells and specific epithelia makes it a crucial target for CAR-T cell therapy against solid tumors.

Targeting Diseases
Colorectal Cancer, Gastric Cancer, Breast Cancer, Ovarian Cancer, Pancreatic Cancer

UniProt ID
P16422

Gene ID
4072

Target Species
Human

Antibody Clone
7-F17

scFv-Host Species
Human

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Customer Reviews and Q&As

There are currently no customer reviews or questions for VivoCAR™ Anti-EPCAM (7-F17) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX32). Click the button below to contact us or submit your feedback about this product.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.