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Anti-Human CD33 (Gemtuzumab) h(41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF22)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-CD33 (Gemtuzumab) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human CD33. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-CD33 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-CD33 (Gemtuzumab) linked to 2nd generation CAR containing 4-1BB and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

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Description

  • Target
  • CD33
  • Components
  • This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
    1. A CAR lentiviral vector
    • Anti-CD33 (Gemtuzumab) h(41BB-CD3ζ) CAR
    2. Three packaging vectors
    • LentiArt™ pHelp1
    • LentiArt™ pHelp2
    • LentiArt™ pHelp3
    3. CAR detection reagent
    4. 293 cells
  • Size
  • 10 reactions; 20 reactions; 50 reactions
  • Storage Conditions
  • For details of the storage requirements, please see the User Manual for instructions.
  • Applications
  • CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

  • CAR Generation
  • 2nd-generation CAR
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-41BB-CD3ζ

Target

  • Gene Name
  • CD33
  • Full Name
  • CD33 Molecule
  • scFv
  • Gemtuzumab
  • Host Species
  • Human
  • Target Species
  • Human
  • Target Background
  • Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
  • Synonyms
  • CD33 Molecule; Sialic Acid-Binding Ig-Like Lectin 3; CD33 Antigen (Gp67); SIGLEC-3; SIGLEC3; Gp67
  • Related Disease
  • Acute myeloid leukemia (AML)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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