All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Creative Biolabs has organized a staff of outstanding scientists who have engaged in Biomarker Development for many years. Scientists at Creative Biolabs are experts in performing a range of Enzyme-linked immunosorbent assay (ELISA) assays for biomarker identification to monitoring immune responses.
Biomarkers have taken a central position either as companion markers to support drug development and patient selection or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, the capabilities to analyze protein biomarkers are highly interesting for biomedical research. The traditional ELISA technique continues to be widely used for the quantitation of a large variety of proteins. The single-plex ELISA, which is mostly performed in 96- or 384-well plates, has played a prominent role in the quantitative and qualitative identification of analytes.
ELISA monitoring is a very simple and convenient way to quantitate immune responses. With an experienced team of ELISA experts, Creative Biolabs offers highly customizable solutions for our customers. To develop the ELISA protocol, several parameters are optimized including antigen concentration, serum dilutions, incubation time, temperature, and dilutions of secondary antibodies.
A two-step ELISA involves a primary antibody and a labeled secondary antibody. This method presents a higher sensitivity and flexibility.
Quantify antigens between the two layers of antibodies.
Competitive ELISA is based on a competitive binding process between the original antigen in the sample and the add-in antigen, the more antigen in the sample, the less labeled antigen is retained in the well and the weaker the signal.
Digital ELISA uses paramagnetic beads coupled to specific antibodies, which are isolated in femtoliter-sized nanowells designed to isolate a single bead in a high-throughput manner. This technology reduces the nonspecific background fluorescent signal and enables signal-molecule detection in a digital fashion.
For more information on ELISA for immune monitoring, please feel free to contact us and further discuss with our scientists.
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