VivoCAR™ Anti-5T4 (H8-C) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX4)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-5T4 (H8-C) h(CD28-41BB-CD3z) CAR circRNA integrates the potent H8 chimeric ScFv into our advanced circular RNA platform to target the oncofetal antigen 5T4 with high structural rigidity. In the context of in vivo CAR-T therapy, the H8 chimeric clone is highly significant for its robust antigen-binding kinetics, which ensures immediate and forceful T-cell activation upon expression in the tumor microenvironment. This product is specifically designed to leverage the high translation efficiency of circRNA, providing a steady pulse of CAR expression that overcomes the immunosuppressive barriers of 5T4-positive solid tumors. It serves as a powerful tool for researchers requiring a highly reactive and reliable "on-switch" for transient in vivo immune redirection against complex carcinomas.

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Target Information

  • Target
  • 5T4
  • Common Name
  • 5T4/Trophoblast Glycoprotein
  • Alternative Names
  • TPBG, M6P1, 5T4AG, WAIF1
  • Full Name
  • Trophoblast Glycoprotein
  • Introduction
  • 5T4 is a highly glycosylated transmembrane protein overexpressed in a variety of solid tumors but minimally expressed in normal adult tissues, making it an excellent target for cancer immunotherapy. It is often associated with tumor metastasis and poor prognosis
  • Targeting Diseases
  • Colorectal Cancer, Renal Cell Carcinoma, Gastric Cancer, Ovarian Cancer, Non-Small Cell Lung Cancer (NSCLC)
  • Target Species
  • Human
  • Antibody Clone
  • H8
  • scFv-Host Species
  • Chimeric:Mouse variable region + human constant region
  • Receptor_Construction
  • CD28-41BB-CD3z
  • Generation
  • Third
  • Description of Signaling Cassetes
  • The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.
  • Structure
  • Circula
  • Product Type
  • CAR IVT circRNA
  • Concentration
  • Lot specific
  • Storage
  • Aliquot and store at -80°C. Avoid freeze/thaw cycles.
  • Handling
  • Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.
  • IVT Vector Sequencing
  • Pass
  • IVT Vector Enzyme Digestion
  • Pass
  • Purity OD260/280
  • Pass
  • RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
  • Pass
  • RNA Sequence Assessment
  • Pass
  • Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
  • Pass
  • Endotoxin (<10 EU/mg)
  • Pass
  • In Vitro Transfection and Expression (Optional)
  • Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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