VivoCAR™ Anti-GPC3 (V209) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX121)

Name: VivoCAR™ Anti-GPC3 (V209) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX121
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-GPC3 (V209) h(CD28-41BB-CD3z) CAR circRNA utilizes the V209 clone to target the oncofetal antigen GPC3 in hepatocellular carcinoma. This clone is significant for its ability to recognize the carboxyl-terminal fragment of GPC3, which is a major driver of Wnt signaling in liver cancer. By employing the circRNA platform, the product delivers a high-fidelity translation window, allowing in vivo generated CAR-T cells to neutralize the liver tumor mass. Its transient profile provides a superior safety margin, protecting healthy tissues while purging GPC3-overexpressing malignant cells.

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Target Information

Target
GPC3

Common Name
GPC3

Alternative Names
DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1

Full Name
Glypican-3

Introduction
GPC3 is a member of the glypican family of heparan sulfate proteoglycans. It is an oncofetal protein that is highly and specifically overexpressed in Hepatocellular Carcinoma (HCC) and other yolk sac tumors, while being virtually absent in healthy adult liver and other tissues. GPC3 plays a crucial role in promoting tumor cell proliferation by modulating the Wnt signaling pathway, making it one of the most promising and clinically advanced targets for CAR-T therapy in solid tumors.

Targeting Diseases
Hepatocellular Carcinoma (HCC), Hepatoblastoma, Yolk Sac Tumors, Squamous Cell Lung Cancer

UniProt ID
P51654

Gene ID
2719

Target Species
Human

Antibody Clone
V209

scFv-Host Species
Human

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.