VivoCAR™ Anti-B7H4 (2D1) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX67)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-B7H4 (2D1) h(CD28-41BB-CD3z) CAR circRNA utilizes the 2D1 clone to neutralize the potent immunosuppressive signals found in the solid tumor microenvironment. In the realm of in vivo engineering, this product is vital for its ability to target B7H4-expressing tumor-associated macrophages and malignant cells simultaneously. By leveraging the circRNA platform, the 2D1-CAR provides a steady translation pulse that empowers endogenous T cells to infiltrate "cold" tumors like breast and ovarian cancer, offering a safe, non-integrating alternative for revitalizing anti-tumor immunity without the risks of viral mutagenesis.

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Target Information

  • Target
  • B7H4
  • Common Name
  • B7H4/VTCN1
  • Alternative Names
  • B7X, VTCN1, B7S1, B7-H4, B7h.5, VCTN1, PRO1291
  • Full Name
  • V-Set Domain Containing T Cell Activation Inhibitor 1
  • Introduction
  • B7-H4 is a member of the B7 superfamily of immune checkpoint molecules. It is an inhibitory ligand expressed mainly on tumor cells and tumor-associated macrophages (TAMs), playing a critical role in promoting tumor immune evasion by suppressing T cell activation and proliferation. Its restricted expression on normal tissues makes it a highly promising target for solid tumor CAR therapy.
  • Targeting Diseases
  • Ovarian Cancer, Breast Cancer, Endometrial Cancer, Lung Cancer
  • Target Species
  • Human
  • Antibody Clone
  • 2D1
  • scFv-Host Species
  • Mouse
  • Receptor_Construction
  • CD28-41BB-CD3z
  • Generation
  • Third
  • Description of Signaling Cassetes
  • The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.
  • Structure
  • Circula
  • Product Type
  • CAR IVT circRNA
  • Concentration
  • Lot specific
  • Storage
  • Aliquot and store at -80°C. Avoid freeze/thaw cycles.
  • Handling
  • Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.
  • IVT Vector Sequencing
  • Pass
  • IVT Vector Enzyme Digestion
  • Pass
  • Purity OD260/280
  • Pass
  • RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
  • Pass
  • RNA Sequence Assessment
  • Pass
  • Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
  • Pass
  • Endotoxin (<10 EU/mg)
  • Pass
  • In Vitro Transfection and Expression (Optional)
  • Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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