VivoCAR™ Anti-FASLG (NOK2) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX41)

Name: VivoCAR™ Anti-FASLG (NOK2) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX41
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-FASLG (NOK2) h(CD28-41BB-CD3z) CAR circRNA incorporates the NOK2 clone to reverse FasL-mediated immune evasion through direct in vivo engineering. The NOK2 clone is significant for its ability to block the apoptotic induction of effector T cells by the tumor, effectively turning a "death signal" into a target for destruction. By employing the circRNA platform, this product provides a transient surge of CAR-T activity that purges FasL-overexpressing malignant cells, restoring a favorable immune balance in the microenvironment without the risks of permanent insertional mutagenesis.

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Target Information

Target
FASLG

Common Name
FASLG

Alternative Names
APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, TNLG1A, APT1LG1

Full Name
Fas Ligand

Introduction
FASLG is a type II transmembrane protein and a member of the TNF family. It binds to its receptor, Fas (CD95), triggering apoptosis. Tumor cells often upregulate FASLG as a mechanism of immune evasion, killing infiltrating anti-tumor T cells. Targeting FASLG-expressing tumor cells is a strategy to eliminate the tumor while potentially reversing this immunosuppressive mechanism.

Targeting Diseases
Various Solid Tumors (e.g., Melanoma, Colon, Lung Cancer)

UniProt ID
P48023

Gene ID
356

Target Species
Human

Antibody Clone
NOK2

scFv-Host Species
Mouse

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.