VivoCAR™ Anti-ALPPL2 (3H2) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX91)

Name: VivoCAR™ Anti-ALPPL2 (3H2) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX91
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-ALPPL2 (3H2) h(CD28-41BB-CD3z) CAR circRNA utilizes the 3H2 clone to target oncofetal alkaline phosphatase with high precision. In in vivo settings, this product is pivotal for identifying and destroying aggressive mesothelioma and testicular cancer cells. By leveraging the circRNA platform, this product achieves significantly higher protein yield than linear mRNA, ensuring that transiently programmed T cells can effectively engage malignant cells while maintaining a safe, non-integrating profile that avoids permanent impact on healthy tissue.

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Target Information

Target
ALPPL2

Common Name
ALPPL2

Alternative Names
GCAP, ALPPL, ALPG

Full Name
Alkaline Phosphatase, Placental-Like 2

Introduction
ALPPL2 is a tissue-nonspecific alkaline phosphatase expressed primarily in the placenta. It is considered an Oncofetal Antigen because it is ectopically expressed on the surface of various malignant tumors, including Ovarian Cancer, Lung Adenocarcinoma, and Germ Cell Tumors. Its highly restricted expression pattern in normal adult tissue makes it an excellent target for CAR-T cell therapy, offering high specificity and minimizing off-target toxicity.

Targeting Diseases
Ovarian Cancer, Testicular Germ Cell Tumors, Lung Cancer (Adenocarcinoma), Bladder Cancer

UniProt ID
P10696

Gene ID
269

Target Species
Human

Antibody Clone
3H2

scFv-Host Species
Mouse

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

VivoCAR™ Anti-ALPPL2 (3H2) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX91)

Target Information

Case Studies

Customer Reviews and Q&As

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