Anti-CEA (hMN14) h(41BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
CEA

Targeting Cell Type
T cell

Targeting Diseases
Others

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-4-1BB-CD3ζ

Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

Customize Your CAR Products

Published Data

CAR scFv data FCM

Fig.1 Identifying MN-14 epitope-containing domain of CEA. FACS profiles of transfectant cells were obtained after staining with humanized anti-CEA Ab hMN-14.

CAR Construction :

Fig.1 Identifying MN-14 epitope-containing domain of CEA. FACS profiles of transfectant cells were obtained after staining with humanized anti-CEA Ab hMN-14.

All other constructs were expressed in LR-73 cells that have normal glycosylation.

Ma, Q., DeMarte, L., Wang, Y., Stanners, C. P., & Junghans, R. P. (2004). Carcinoembryonic antigen-immunoglobulin Fc fusion protein (CEA-Fc) for identification and activation of anti-CEA immunoglobulin-T-cell receptor-modified T cells, representative of a new class of Ig fusion proteins. Cancer gene therapy, 11(4), 297-306.

CAR scFv data FCM

Fig.2 Identification of MN-14 antibody and Fig.3 Anti-CEA IgTCR by CEA-Fc.

CAR Construction :

Fig.2 Identification of MN-14 antibody and Fig.3 Anti-CEA IgTCR by CEA-Fc.

Anti-CEA IgTCR chimeric receptor was detected by flow cytometric analysis using CEA-Fc.

CAR scFv data FCM

Fig.3 Activation of anti-CEA IgTCR-transduced Jurkat T cells by CEA-Fc.

CAR Construction :

Fig.3 Activation of anti-CEA IgTCR-transduced Jurkat T cells by CEA-Fc.

Jurkat and anti-CEA IgTCR-transduced Jurkat cells (Jurkat-IgTCR) were incubated for 24 hour with BSA, OKT3, CEA, and CEA-Fc in soluble form.

Complete CAR data FCM

Fig.4 CD8+ T cells were transduced with the retroviral constructs and GFP+ CD8+ T cells (upper panel) or GFP+ Myc+ T cells gated on GFP+ CD8+ populations were analyzed by flow cytometry

CAR Construction : CEA scFv-TM28-CD28-CD3ζ

Fig.4 CD8+ T cells were transduced with the retroviral constructs and GFP+ CD8+ T cells (upper panel) or GFP+ Myc+ T cells gated on GFP+ CD8+ populations were analyzed by flow cytometry

In vitro generation of carcinoembryonic antigen (CEA) chimeric antigen receptor T (CAR-T) cells.

Fan, J., Das, J. K., Xiong, X., Chen, H., & Song, J. (2021). Development of CAR-T cell persistence in adoptive immunotherapy of solid tumors. Frontiers in oncology, 10, 574860.

Complete CAR data IB

Fig.5 The packaging Plat-E cells (upper panel) and MFG-transduced CD8+ T cells (lower panel) were visualized by fluorescence microscope.

CAR Construction : CEA scFv-TM28-CD28-CD3ζ

Fig.5 The packaging Plat-E cells (upper panel) and MFG-transduced CD8+ T cells (lower panel) were visualized by fluorescence microscope.

scale bars: 200 mm

Fan, J., Das, J. K., Xiong, X., Chen, H., & Song, J. (2021). Development of CAR-T cell persistence in adoptive immunotherapy of solid tumors. Frontiers in oncology, 10, 574860.

Complete CAR data WB

Fig.6 GFP+ CD8+ T cells were sorted, and the cell lysates were determined for the expression of Bcl-xL and b-actin by
western blotting.

CAR Construction : CEA scFv-TM28-CD28-CD3ζ

Fig.6 GFP+ CD8+ T cells were sorted, and the cell lysates were determined for the expression of Bcl-xL and b-actin by western blotting.

All data are representative of three independent experiments.

Fan, J., Das, J. K., Xiong, X., Chen, H., & Song, J. (2021). Development of CAR-T cell persistence in adoptive immunotherapy of solid tumors. Frontiers in oncology, 10, 574860.

Complete CAR data Cyt

Fig.7 Carcinoembryonic antigen (CEA) chimeric antigen receptor T (CAR-T) cells overexpressing Bcl-xL had modest in vitro cytotoxicity.

CAR Construction : CEA scFv-TM28-CD28-CD3ζ

Fig.7 Carcinoembryonic antigen (CEA) chimeric antigen receptor T (CAR-T) cells overexpressing Bcl-xL had modest in vitro cytotoxicity.

Representatives of the in vitro co-culture of T cells with MC-32 tumor cells at 12 h (scale bars: 50 mm).

Fan, J., Das, J. K., Xiong, X., Chen, H., & Song, J. (2021). Development of CAR-T cell persistence in adoptive immunotherapy of solid tumors. Frontiers in oncology, 10, 574860.

Complete CAR data FuncS

Fig.8 Representatives of the CD8+ Thy1.2+ T cells in the LNs and spleen on day 14.

CAR Construction : CEA scFv-TM28-CD28-CD3ζ

Fig.8 Representatives of the CD8+ Thy1.2+ T cells in the LNs and spleen on day 14.

All data are representative of three independent experiments.

Fan, J., Das, J. K., Xiong, X., Chen, H., & Song, J. (2021). Development of CAR-T cell persistence in adoptive immunotherapy of solid tumors. Frontiers in oncology, 10, 574860.

More Published Data More Published Data

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-CEA (hMN14) h(41BB-CD3ζ) CAR, pCDCAR1 (CAR-ZP015)

Specific Inquiry

Details

Target

Customize Your CAR Products

Customer Reviews and Q&As

Related Products

CAR Vector Products

CAR Cell Products

CAR Viral Particles

CAR Animal Cells