Close

pCDCAR1 CLEC12A h(BBζ, α/β/γ) (CAR-LC334)

The vector of anti-CLL-1 chimeric antigen receptor (CAR) is based on the FcεRI receptor scaffold and constructed for the engineering of T cells to target human CLL-1. The T cells are genetically modified through transduction with a lentiviral vector expressing an alpha chain comprising a scFv of anti-CLL-1 antibody linked to FcεR1γ-SP and FcεR1γ-TM-IC, a beta chain comprising FcεR1γ-ΔITAM linked to 4-1BB (CD137) signaling domain, a gamma chain comprising FcεR1γ-SP-ΔITAM linked to CD3ζ signaling domain. And the vector product was designed for the treatment of acute lymphoblastic leukemia.

Details

  • Target
  • CLEC12A
  • Target Species
  • Human
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • Acute lymphoblastic leukemia
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral
  • Receptor Construction
  • FcεR1γ-SP-scFv-FcεR1γ-TM-IC/FcεR1γ-ΔITAM-41BB/FcεR1γ-SP-ΔITAM-CD3ζ
  • Discription of Signaling Cassetes
  • 41BB
    CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
  • Antibody Clone
  • 21.26
  • Antibody Host
  • Mouse

Target

  • Gene Name
  • C-type lectin domain family 12, member A
  • Official Symbol
  • CLEC12A
  • Synonyms
  • CLEC12A; C-type lectin domain family 12; member A; CLL1; MICL; CLL-1; DCAL-2; C-type lectin domain family 12 member A; C-type lectin protein CLL-1; C-type lectin-like molecule-1; dendritic cell-associated lectin 2; myeloid inhibitory C-type lectin-like receptor; anti-CLEC12A

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Related Products

Online Inquiry
Products

CONTACT US

45-1 Ramsey Road, Shirley, NY 11967, USA
Call us at:
USA: 1-631-381-2994
Europe: 44-207-097-1828
Fax: 1-631-207-8356
Email:

Online Inquiry

For any technical issues or products/services related questions, please leave your contacts as below, and our team will contact you at earliest convenience to let you know how we can be involved in your projects.

  • Verification code

Key Updates

Receive the latest news and insights to your inbox.