VivoCAR™ Anti-MUC16 (11D10) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX52)

Name: VivoCAR™ Anti-MUC16 (11D10) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX52
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-MUC16 (11D10) h(CD28-41BB-CD3z) CAR circRNA focuses on targeting the proximal extracellular domain of MUC16, which remains on the cell surface after the CA125 fragment is shed. For in vivo applications, the 11D10 clone is critical for circumventing the "decoy effect" of circulating soluble antigens. The circRNA platform ensures a stable and durable expression of the CAR on endogenous lymphocytes, facilitating the persistent destruction of residual ovarian cancer cells in the peritoneal cavity without the risks associated with permanent viral gene delivery.

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Target Information

Target
MUC16

Common Name
MUC16

Alternative Names
Mucin16, Ovarian Cancer Antigen CA-125, CA125

Full Name
Mucin 16, Cell Surface Associated

Introduction
MUC16 is a very large, heavily glycosylated mucin protein highly expressed on ovarian cancer cells and several other adenocarcinomas. The shedding of its extracellular domain is measured as CA-125 in serum. The large, extracellular domain provides numerous accessible epitopes, making it a well-established and essential target for CAR-T cell therapy, especially for peritoneal malignancies.

Targeting Diseases
Ovarian Cancer, Pancreatic Cancer, Endometrial Cancer, Non-Small Cell Lung Cancer (NSCLC)

UniProt ID
Q8WXI7

Gene ID
94025

Target Species
Human

Antibody Clone
11D10

scFv-Host Species
Mouse

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.