Given its important role in cancer progression and development, activated leucocyte CAM (ALCAM) has drawn significant scientific interest over the past years. ALCAM has significant potential as a fluid-based biomarker because the shed ectodomain of ALCAM is released into adjacent biofluids. Throughout our years of providing biomarker analysis services to global clients, Creative Biolabs has established ongoing working relationships with experts in the field of antibody development, assay development, and validation, allowing us to offer advanced custom services at a level of quality that clients cannot find anywhere else.
ALCAM is a 105 kDa transmembrane glycoprotein that belongs to the immunoglobulin super family (IgSF). Soluble ALCAM in body fluids is produced through proteolytic cleavage by ADAM17 and MMP14. The major function of ALCAM includes mediating immune cell adhesion and migration and promoting T cell activation and proliferation.
Fig.1 ALCAM structure and interactions between and within cells. (Yang, 2021)
ALCAM has been increasingly used as a biomarker for the diagnosis, treatment response, and survival prediction in various cancers. ALCAM was validated and identified as a novel alternate surface marker on EpCAMlow CTCs. In type 2 diabetic nephropathy, serum ALCAM level and ALCAM expression in the glomeruli were significantly increased. Besides, urinary ALCAM is also a promising non-invasive biomarker for renal histopathology in Lupus nephritis (LN). Figure 2 demonstrates the available information on ALCAM protein concentrations in the pan-cancer cohort.
Fig.2 ALCAM protein concentrations in the pan-cancer cohort.
Creative Biolabs provides ALCAM detection assays at both the gene level and protein level. We have established validated methods for quantitation of ALCAM. Generally, shed ALCAM in serum and urine could be measured by ELISA while ALCAM shedding in tissues could be detected through immunohistochemical approach. Our popular analysis service covers for instance:
| Cat | Service |
| BAS85-1 | ALCAM mRNA Expression Analysis |
| BAS85-2 | ALCAM Protein Expression Analysis |
| BAS85-3 | RNA Interference Assay |
| BAS85-4 | LFA-based Antibody Screening |
| BAS85-5 | ALCAM LFA strips Development |
| BAS85-6 | Quantitative Analysis of ALCAM Shedding |
Creative Biolabs puts to use our years of experience in testing and analysis to provide the best data and help you understand what it means. If you are interested in our services, please don't hesitate to contact us today to develop custom solutions for specific needs. We will work to accommodate your request.
Reference
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