Screen and profile small molecules and biologics to identify novel NAMPT inhibitors for cancer and metabolic disease research.
Are you currently facing challenges in identifying and validating drug targets or developing effective inhibitors for metabolic diseases and oncology? Our comprehensive nicotinamide phosphoribosyltransferase (NAMPT) analysis services help you accelerate your drug discovery and diagnostic projects by providing precise, high-quality data through our advanced assay platforms and expert consultation.
Introduction of NAMPT
NAMPT is a metabolic enzyme that catalyzes the rate-limiting step in the NAD⁺ salvage pathway. It is a highly relevant biomarker and therapeutic target, particularly in oncology, as its expression is significantly elevated in various cancers, contributing to tumor proliferation and drug resistance. Research has shown that NAMPT plays a critical role in cellular energy metabolism and stress response.
Fig.1 Biological functions of NAMPT.1
Simple Workflow
01Initial Consultation and Experimental Design
Our experts will review your project requirements to design a tailored NAMPT analysis strategy. This step ensures that the methodology aligns perfectly with your research objectives.
02Sample Preparation and Assay Setup
Your provided materials are carefully prepared, and a suitable NAMPT assay is configured. We utilize a highly sensitive fluorescent-based assay for screening and profiling NAMPT inhibitors.
03High-Throughput Screening
We screen your compound library against the NAMPT target using our automated platforms. This high-throughput approach rapidly identifies potential hit compounds that inhibit NAMPT activity.
04Data Analysis and Validation
Raw data from the screening process is meticulously analyzed to determine IC50 values and identify promising leads. This step includes running positive and negative controls to validate the assay's performance.
05Comprehensive Reporting
You will receive a detailed report outlining the experimental protocols, raw data, analyzed results, and a summary of key findings.
Key Highlights
At Creative Biolabs, our NAMPT analysis services stand apart due to our cutting-edge technology, unparalleled expertise, and commitment to delivering high-quality, actionable data. We leverage advanced assay platforms and an integrated workflow that ensures reliable and reproducible results, helping you overcome critical roadblocks in your research.
Applications
Our NAMPT analysis services are designed to support a wide range of applications in both research and commercial settings.
Drug Discovery & Development
Target Validation
Confirm the role of NAMPT in specific disease pathways and validate it as a viable drug target.
Biomarker Analysis
Use NAMPT levels and activity as a prognostic biomarker to monitor disease progression or predict treatment response.
Mechanism of Action Studies
Investigate how your compound interacts with NAMPT to better understand its therapeutic potential.
Assay Development
Obtain high-quality recombinant NAMPT protein to support the development of your own in-house assays.
FAQs
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What is the main application of your NAMPT analysis services?
Our NAMPT analysis services are primarily used for high-throughput screening of inhibitor compounds, target validation, and understanding the role of NAMPT in disease pathways. Our solutions are designed to support drug discovery and diagnostic development projects.
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How does your NAMPT analysis differ from other commercial kits?
While some commercial kits provide basic components, our service offers a comprehensive solution. This includes expert consultation, a customizable workflow tailored to your specific project needs, and a detailed final report with in-depth data analysis and interpretation. We handle the entire process, saving you time and resources.
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What is the turnaround time for a typical NAMPT inhibitor screening project?
The estimated timeframe is generally 2-4 weeks after we receive your samples. This may vary based on the complexity of your project and the size of the compound library you wish to screen. For more specific project timelines, we encourage you to contact us for a detailed quote.
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What level of sensitivity can I expect from your NAMPT analysis?
Our fluorescent-based NAMPT assay is highly sensitive, designed to accurately detect even subtle changes in enzyme activity. This allows for precise determination of IC50 values and the identification of low-affinity hit compounds, ensuring no potential leads are missed.
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Can your services be used for both small molecules and biologics?
Yes, our versatile NAMPT analysis platform can be adapted to screen various compound types, including small molecules and biologics. Our scientific team can design a custom assay to meet the unique requirements of your research.
Related Services
Recombinant Protein Expression
Obtain high-quality, biologically active recombinant NAMPT protein for your in-house assays and experiments.
NAD⁺/NADH Assay Services
Quantify NAD⁺ and NADH levels to assess metabolic health and pathway activity downstream of NAMPT.
Drug Discovery & Screening
Leverage our expertise for broader high-throughput screening projects across various other therapeutic targets.
Creative Biolabs provides a complete solution for NAMPT analysis, from expert-led consultation and a highly efficient workflow to comprehensive data delivery. Our services are tailored to accelerate your research and provide reliable, actionable results. To learn more about how our NAMPT analysis services can be tailored to meet your specific project needs and to receive a detailed quote, please reach out to our team of experts.
Reference
- Peng, Aihong, et al. "The function of nicotinamide phosphoribosyl transferase (NAMPT) and its role in diseases." Frontiers in Molecular Biosciences 11 (2024): 1480617. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fmolb.2024.1480617
For Research Use Only.
