Can your ZFP36L1 analysis services differentiate between protein levels and functional activity?

Our ZFP36L1 analysis services are designed to differentiate between protein levels and functional activity. We integrate multiple techniques such as Western blotting for protein quantification with functional assays like Luciferase reporter assays to measure the biological impact of ZFP36L1.

Is ZFP36L1 analysis suitable for both tumor-suppressive and oncogenic roles?

ZFP36L1 analysis is suitable for investigating both tumor-suppressive and oncogenic roles. The analytical approach is tailored to the specific context, allowing us to evaluate its expression and function, whether it is downregulated as a tumor suppressor or overexpressed in a paradoxical oncogenic role.

How do you account for post-translational modifications of ZFP36L1 in your analysis?

We account for post-translational modifications of ZFP36L1 in our analysis by employing advanced proteomic and functional assays. These methods allow us to investigate phosphorylation states and other modifications that critically impact ZFP36L1's stability and activity.

Can your services help identify new ZFP36L1 target mRNAs?

Our services can help identify new ZFP36L1 target mRNAs. We utilize advanced techniques like RNA-sequencing and iCLIP to systematically screen for and validate new mRNA targets regulated by ZFP36L1 in your specific biological model.

Do your services include bioinformatics analysis of the data?

Our services include comprehensive bioinformatics analysis of the data. This analysis allows us to interpret the large datasets generated from our services, such as gene expression profiling and functional assays, to provide a clear understanding of the affected biological pathways.

Can your analysis be customized for different species?

Our analysis can be customized for different species. While our primary focus is on human ZFP36L1, our expertise in assay development allows us to adapt our analytical platforms for animal models or other species based on your specific research requirements.

ZFP36L1 Analysis Service - Creative Biolabs

Creative Biolabs provides our biotech and pharmaceutical partners with fully integrated biomarker services from individual assay to solution optimization and biomarker identification in biology and beyond. With extensive experience in assay development using biochemical and cell-based phenotypic functional assays, scientists at Creative Biolabs are fully equipped to provide a broad array of services for zinc finger protein 36 like 1 (ZFP36L1) analysis.

Introduction of ZFP36L1

The ZFP36L1 gene is remarkably conserved across species, featuring a promoter with motifs similar to other early-response genes. Mechanistically, ZFP36L1 functions by binding to specific AU-rich elements (AREs) located in the 3'-untranslated regions (3'-UTRs) of its target messenger RNAs (mRNAs). This binding initiates mRNA decay, effectively silencing gene expression at the post-transcriptional level and providing a powerful mechanism for fine-tuning cellular responses.

As a member of the Tristetraprolin (TTP) RNA-binding protein family, ZFP36L1 regulates gene expression post-transcriptionally by promoting mRNA degradation. The protein has been shown to be critically involved in controlling cell differentiation, the cell cycle, and apoptosis. Deficiencies in ZFP36L1 and its paralog ZFP36L2 have been shown to significantly increase cell proliferation and cytokine production, highlighting its essential role as a regulator of cellular homeostasis.

The proposed model for the HIF-1α/miR-182-5p/ZFP36L1 signaling in NPC tumorigenesis and metastasis. (OA Literature)

Fig.1 The oncogenic role of HIF-1α/miR-182-5p/ZFP36L1 signaling pathway in NPC.¹

ZFP36L1 Analysis Services at Creative Biolabs

At Creative Biolabs, our ZFP36L1 analysis services are designed to overcome the limitations of standard assays by providing a comprehensive, multi-faceted view of this complex protein. Our integrated approach helps scientists gain critical insights into ZFP36L1’s function in disease and explore the correlation between its expression and patient prognosis. We go beyond basic measurement to assess the protein's functional state, offering a suite of advanced analytical solutions including:

We design, validate, and qualify bespoke assays to address your unique research questions.

We have developed high-specificity ZFP36L1 monoclonal and polyclonal antibodies for the in vitro quantitative measurement of human ZFP36L1 concentrations in various biological fluids.

We use next-generation sequencing to not only quantify ZFP36L1 expression but also to identify and validate its specific mRNA targets.

Our services include immunoprecipitation (IP), Western blot (WB), and immunohistochemistry (IHC) to determine ZFP36L1 abundance and cellular localization.

To identify and validate ZFP36L1's binding partners, we employ advanced methods such as RNA pull-down assays and iCLIP.

We employ advanced assays such as FACS and Lentivirus knockdown studies to measure the functional consequences of ZFP36L1 modulation.

Service Workflow

Our ZFP36L1 analysis workflow is a precise, step-by-step process designed for scientific rigor and client success.

01Initial Consultation

We begin with a detailed discussion to understand your specific research goals, project requirements, and the initial materials you can provide (e.g., cells, tissues, or lysates).

02Custom Protocol Design

Our expert team designs a bespoke ZFP36L1 analysis plan tailored to your needs, ensuring the most accurate and efficient approach, whether it's through qRT-PCR, iCLIP, or other advanced techniques.

03Sample Submission

Clients then provide the necessary starting materials according to our clear guidelines. Our team will handle them with the utmost care, ensuring sample integrity throughout the process.

04Data Generation & Analysis

We perform the agreed-upon ZFP36L1 analysis using our state-of-the-art platforms. Throughout this phase, we maintain transparent communication and provide regular progress updates.

05Final Report Delivery

The project culminates in a comprehensive, detailed report. This includes all methodologies, raw data, quality control results, and a clear interpretation of the findings, giving you actionable insights for your research.

Applications

Oncology Research

ZFP36L1's dual role as a tumor suppressor and, in some contexts, a tumor promoter, makes its expression and functional analysis essential for understanding cancer progression. Its analysis is key for identifying novel therapeutic targets and biomarkers for patient stratification, particularly in breast and colorectal cancers where its downregulation is frequently observed.

Immunology and Inflammation

ZFP36L1 plays a critical role in immune cell regulation. Analyzing its expression and targets is vital for understanding inflammatory disorders, such as asthma, and for developing new immunomodulatory drugs.

Virology

Given its function in innate immunity, ZFP36L1 analysis is an emerging area in virology research. By modulating the host's response to viral infections, ZFP36L1 presents a potential target for developing new antiviral therapies.

Metabolic Disorders

Emerging research links ZFP36L1 to the regulation of key metabolic pathways. Its analysis is increasingly being utilized to investigate conditions like alcoholic hepatic steatosis and other metabolic disorders.

Service Highlights

FAQs

If you are interested in our analysis services at Creative Biolabs, please feel free to contact us for more information.