Adiponectin (ADIPOQ) is intensely researched as a biomarker to predict the risk of some diseases. Creative Biolabs provides comprehensive ADIPOQ analysis services for worldwide clients. The bioanalytical laboratory at Creative Biolabs can develop and validate methods to analyze ADIPOQ in various matrixes such as cell supernatant, plasma, serum, urine, and tissue. Over the past years, we have performed bioanalytical method development for different biomarkers by expression analysis, protein interaction analysis, gene mutation analysis, polymorphisms analysis, and so forth.
Introduction
ADIPOQ is an abundant protein hormone that belongs to a family of adipokines. It is expressed mostly by adipocytes and exists as the most abundant adipokine in human plasma. ADIPOQ is an important regulator of lipid and glucose metabolism. It is an insulin-sensitizing hormone with anti-diabetic, anti-inflammatory, and anti-atherogenic properties. Human ADIPOQ exists in three multimeric forms. Among them, high-molecular-weight (HMW) is thought to represent the major active form of ADIPOQ.
Fig.1 Structural and signaling roles of ADGRL1.1
Polymorphisms in ADIPOQ gene have been associated with breast cancer, adiposity, type 2 diabetes (T2D), and gestational diabetes mellitus (GDM). The ADIPOQ rs266729 G/C gene polymorphism is significantly associated with low ADIPOQ protein levels and linked to susceptibility to develop cancer. Recent studies have also indicated that plasma ADIPOQ levels are inversely correlated with body mass index and insulin resistance. Decreased ADIPOQ serum levels are linked with tumor development and progression and are inversely associated with markers of inflammation.
Diagnostic Power
Among the variations of the ADIPOQ gene reported such as rs182052, rs266729, and rs3774262, the SNP rs266729 which is found within the promoter region is significantly associated with ADIPOQ level and is thought to be linked to susceptibility to cancer-based on its role in influencing serum adiponectin levels. In mammals, dysregulation of ADIPOQ contributes to the pathogenesis of a diverse array of chronic diseases such as fatty liver disease, cardiovascular diseases, and aging. Cellular and animal studies demonstrate that the simultaneous loss of ALDH2 and ADIPOQ activities leads to an increase in cellular formaldehyde sensitivity and multisystem abnormalities including hematopoietic failure.
Fig.2 Diagrammatic representation of the ADIPOQ gene and its regulatory promoter region.
We Have Your Biomarker Assay Needs to be Covered
Creative Biolabs offers vast and complex customized solutions for ADIPOQ analysis at the gene level or protein level through the development and validation of laboratory assays that allow the quantification and characterization of immune responses. Precise data obtained from the biomarker services provide a valuable tool to determine the mechanism of action (MOA) and/or the target engagement of the therapeutic mitigating the risk at key stages of drug development.
Our Service Include but are not limited to:
- Sandwich ELISA
- Chemiluminescent immunoassay
- RNA and gene expression analysis
- Analysis of DNA polymorphisms
- Methylation Analysis
If you are interested in our services, please contact us for more information.
Reference
- Barbe, Alix, et al. "Mechanisms of adiponectin action in fertility: an overview from gametogenesis to gestation in humans and animal models in normal and pathological conditions." International journal of molecular sciences 20.7 (2019): 1526. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
