Biomarker assays are indispensable tools for validating targets, supporting the mechanism of action, predicting therapeutic efficacy, and alerting for potential toxicity. Comprehensive portfolio at Creative Biolabs provides analysis services and high-quality products, scientific, and regulatory experts to fully support your integrated program from start to finish. Find the ATP-binding cassette sub-family G member 8 (ABCG8) analysis service that fits your needs.
Background
ABCG8 is located on chromosome 2p21 and has 13 exons that encode 673 amino acid residues. As a member of the superfamily of ATP-binding cassette (ABC) transporters, ABCG8 plays an important role in the regulation of intestinal plant sterol absorption by excreting plant sterols.
Fig.1 Structure and SNP of ABCG5/G8.1
The ABCG5/ABCG8 transporters are functional heterodimers that are co-expressed in the canalicular membrane of the hepatocyte and the apical membrane of the enterocytes. They serve to export cholesterol and phytosterols across both of these membranes, thereby excluding them from circulation. Formation of a heterodimer is an absolute necessity to direct the ABCG5/ ABCG8 heterodimer from the endoplasmic reticulum to the apical membrane. Mutations in either of these half-transporters result in defective biliary sterol transport, leading to the human disease sitosterolemia.
Scientific Validity
Serum cholesterol-standardized campesterol and sitosterol concentrations, as well as their changes after consumption of plant stanol-enriched foods, are related to a variation in the ABCG8 gene that is present in ∼70% of the population. More and more scientists focus on the relationships between changes in serum plant sterol concentrations with ABCG8 polymorphisms after the consumption of plant stanol esters.
Services at Creative Biolabs
In academic research related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function, and interaction with ABCG5. Creative Biolabs has validated our self-developed anti-ABCG8 antibodies on WB, ELISA, IHC, ICC, and IF with known positive and negative samples to ensure specificity and high affinity. Our popular services targeting ABCG8 include but are not limited to the following:
Fig.2 Services at Creative Biolabs.
If you are interested in our analysis services or need a tailored solution, please feel free to contact us for more information.
Reference
- Xavier, Bala M., et al. "Transmembrane polar relay drives the allosteric regulation for ABCG5/G8 sterol transporter." International journal of molecular sciences 21.22 (2020): 8747. Distributed under Open Access license CC BY 4.0, without modification.
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