Microtubule actin crosslinking factor 1 (MACF1) is involved in multiple cellular functions and is the molecular basis for many degenerative diseases. Creative Biolabs solves complex issues for MACF1 qualitative and quantitative analysis. Whether you need intact protein analysis, assistance with solving matrix issues for your approved therapeutics, or those in-development, or characterization of biologics, we can help.
Background
MACF1, a protein scaffold of more than 700 kDa, coordinates the crosstalk between actin microfilaments and microtubules to support cell-cell connections, cell polarity, vesicular transport, proliferation, and cell migration. Structurally, MACF1 comprises a variable N-terminal domain, central region composed of a plakin domain and a spectrin repeat-containing rod domain and a C-terminal MT-binding domain.
Six different MACF1 isoforms produced via alternative splicing and differential promoter usage have been identified, including MACF1a1, MACF1a2, MACF1a3, MACF1-4, MACF1b, and MACF1c. Unique arrangements of these motifs by RNA splicing or transcription start site variation confers to each isoform its own functional characteristics.
Fig.1 MACF1 signaling in physiological and cancer contexts.1
Aberrant expression of MACF1 is also related to various human cancers. Further investigation using proteomics techniques identified MACF1 as a potential oncofetal biomarker that may be used for the diagnosis of colorectal cancer, pancreatic cancer, non-small cell lung cancer.
Services at Creative Biolabs
Many biological assays use antibodies to detect MACF1 such as WB, ELISA, FCM, IHC. Creative Biolabs uses mainly mouse and rabbit to develop MACF1 antibodies. We validate the specificity of these antibodies to MACF1 by testing them on tissues known to express MACF1 positively and negatively.
Moreover, using our various powerful methods, Creative Biolabs provides MACF1 isoform analysis service for our clients. Our targeted mass spectrometry-based methods can be used as antibody free validation tools as well as used as potential tools to selectively analyze specific protein isoforms. Creative Biolabs also offers mutation identification services by Sanger sequencing and PCR. If you cannot find an individual assay that fit your needs, contact us for a custom plan.
Reference
- Cusseddu, Rebecca, Amélie Robert, and Jean-François Côté. "Strength through unity: the power of the mega-scaffold MACF1." Frontiers in Cell and Developmental Biology 9 (2021): 641727. Distributed under Open Access license CC BY 4.0, without modification.
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