By reducing intracellular drug accumulation, ABCC1 enables tumor survival. It also effluxes tyrosine kinase inhibitors, complicating targeted therapies.
ABCC1 (ATP-Binding Cassette Subfamily C Member 1) is a pivotal ATP-dependent efflux transporter belonging to the ABC superfamily. Its primary function is to protect cells by exporting xenobiotics, chemotherapeutic agents, and endogenous signaling molecules. Overexpression in cancers such as breast, lung, and ovarian tumors correlates with poor prognosis due to multidrug resistance (MDR). Beyond oncology, ABCC1 regulates neurotransmitter clearance, heavy metal detoxification, and inflammatory responses, linking it to neurodegenerative diseases, asthma, and environmental toxicity.
Fig.1 Schematic diagram of the secondary structure of human MRP1 highlighting CL4.1
ABCC1's influence spans cellular defense, signaling, and homeostasis:
By reducing intracellular drug accumulation, ABCC1 enables tumor survival. It also effluxes tyrosine kinase inhibitors, complicating targeted therapies.
Transports prostaglandin E2 and leukotriene C4, exacerbating airway inflammation in asthma and joint inflammation in rheumatoid arthritis.
Clears neurotoxic metabolites across the blood-brain barrier, offering potential in Alzheimer's and Parkinson's disease research.
Exports arsenic and cadmium conjugates, protecting against environmental toxicity.
Modulates glutathione homeostasis, influencing cellular redox status and apoptosis.
Our ABCC1 analysis service leverages a range of advanced, state-of-the-art methodologies to comprehensively decode the functions of ABCC1.
We employ cutting-edge gene editing techniques to precisely perform knockout or knock-in of the ABCC1 gene in diverse cell lines and transgenic mice. This facilitates in-depth exploration of ABCC1's role and the impacts of its genetic modification at both cellular and organismal levels, creating tailored models for detailed study.
Our high-throughput screening platform is used to swiftly identify ABCC1 modulators, including small molecules and biological agents that can affect its function. We also offer custom screens to find factors contributing to drug resistance.
Using highly specific antibodies, we accurately quantify ABCC1 protein levels in various samples, assessing changes under different experimental conditions.
This technique maps ABCC1 localization in FFPE or frozen tissue sections, providing insights into its expression patterns in different cell types and tissue compartments.
With PE-conjugated antibodies, we measure membrane-bound ABCC1 in live cells, offering quantitative data on its surface expression and enabling analysis of heterogeneous cell populations.
UPLC-MS/MS precisely quantifies ABCC1 substrates and maps its interaction networks, revealing metabolic changes associated with its activity and the substrates it transports.
Proteomic profiling identifies ABCC1-interacting proteins, uncovering new regulatory mechanisms and potential therapeutic targets, as well as post-translational modifications that may influence its function.
Our 3D and organoid models, using patient-derived organoids (PDOs), realistically mimic the tumor microenvironment. This allows us to study ABCC1-mediated drug resistance in a more physiologically relevant context, investigating how the tumor microenvironment influences ABCC1 expression and function.
ABCC1 analysis aids in cancer research by identifying its expression in cancers like triple-negative breast cancer. It helps screen inhibitors to reverse multidrug resistance, enhancing chemotherapy efficacy.
ABCC1 is involved in amyloid-beta clearance in Alzheimer's. Analysis of its role guides therapeutic strategies to slow disease progression. It also optimizes CNS drug delivery by identifying compounds that utilize or bypass ABCC1 transport.
ABCC1 analysis is crucial for understanding drug ADME processes, especially in tissues with high expression. It predicts drug-drug interactions in polypharmacy, ensuring safety and efficacy by avoiding altered drug levels or toxicity.
ABCC1 affects dendritic cell function in antigen presentation. Analysis guides immunotherapy development, like cancer vaccines, by modulating ABCC1 to enhance immune responses against tumors or pathogens.
Our team boasts leading ABCC1 researchers with extensive experience. We utilize cutting-edge tools like high-throughput sequencing and advanced mass spectrometry. These enable precise and comprehensive ABCC1 analysis, offering reliable data for informed decisions. For instance, our high-throughput platforms can swiftly screen for ABCC1 inhibitors from large libraries, saving time in drug discovery.
We know each project is unique. So, we offer customized ABCC1 analysis. Whether it's studying ABCC1 in a specific tissue, its role in cancer drug resistance, or the impact of toxins on its function, we'll design a plan just for you.
We don't just give raw data. Our bioinformaticians and molecular biologists analyze it using advanced stats and tools. Then, we present clear findings, highlighting key insights and their implications.
Time is crucial in research. We promise fast turnaround times. Our efficient project management keeps your project on track. We've streamlined processes to reduce delays and keep you updated. For a basic ABCC1 expression analysis, we can finish it in a few weeks, depending on complexity.
Quality is our priority. We have strict quality control at every step, from sample handling to data analysis. Our labs have the latest systems, and staff follow standard procedures. We also protect your data's confidentiality and security, giving you confidence in the results for research or regulatory use.
We value long-term client relationships. Our customer support team is always ready to answer questions, offer technical help, and address concerns. We encourage collaboration and welcome your feedback.
We offer HEK293T, HeLa, MCF7, HepG2, U87, and patient-derived xenograft (PDX) cells. Custom lines can be engineered.
Protein: Western blot and IHC;
mRNA: qRT-PCR with probes.
No. All antibodies and kits are strictly for research use only (RUO) and not approved for diagnostics.
ABCC1's dual role in physiology and pathology makes it a high-value target for drug discovery and biomarker development. Our ABCC1 analysis service provides the tools to dissect its mechanisms, overcome resistance, and pioneer personalized therapies. Contact us today to explore customized solutions.
Reference
For Research Use Only.