ADIPOR1 Analysis - Creative Biolabs

Biomarker analysis services provide powerful approaches to measure biological process indicators in normal and pathological conditions or response to pharmacological intervention. As a nimble biomarker CRO, Creative Biolabs offers singleplex or multiplex assays for biomarker analysis. All these assays undergo a fit for purpose biomarker validation, including assessing bioanalytical parameters such as precision, accuracy, and sensitivity. Creative Biolabs now provides a comprehensive set of adiponectin receptor 1 (ADIPOR1) analysis services for research use.

Introduction

Adiponectin exhibits its functions through the adiponectin receptors ADIPOR1 and ADIPOR2. ADIPOR1 is expressed ubiquitously and ADIPOR2 is predominant in the liver. ADIPOR1 consists of a seven-transmembrane domain that is coupled to a G protein signaling pathway. The ADIPOR1 receptor is unique in that the C-terminal end is located on the extracellular side of the membrane and N-terminal on the intracellular side.

Fig.1 ADIPOR1 structure. (Pugia, 2009)

Diagnostic Power

ADIPOR1 overexpression reduces lipid accumulation and hypertrophy in the heart of diet-induced obese mice which indicated its possible involvement of oxidative stress and autophagy.
Studies indicate that ADIPOR1 receptor expression is reduced in diabetic conditions. The relative distribution of ADIPOR1 receptor fragments in the plasma could be regarded as a biomarker for diabetes.
Besides, reported studies also suggest that the ADIPOR1 rs1342387(G/A) polymorphism is significantly associated with the risk of colorectal cancer among individuals of Asian ancestry.
ADIPOR1 rs7539542 has also been associated with insulin resistance in patients with type 2 diabetes (T2D).

Fig.2 Translational research targeted adiponectin and ADIPORs. (Yamauchi, 2013)

We Have Your Biomarker Assay Needs Covered

Using WB and ELISA, fragments of the extracellular C terminus of ADIPOR1 could be detected in human plasma and urine. Having the monoclonal antibody against ADIPOR1 C-terminal fragment (CTF) will allow the development of specific immunoassays to be used in assessing the utility of this putative biomarker.

As a CRO with great experience in antibody expression, Creative Biolabs has developed high-quality monoclonal ADIPOR1 antibodies suitable for the detection of the ADIPOR1 protein or its CTF of the mouse, rat, and human origin. Antibody development is offered as an integrated service end-point or as a stand-alone option.