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VivoCAR™ Anti-CD20 (GA101) h(NKG2D-2B4-CD3z) CAR-NK, mRNA-LNP (XS-0826-LX15)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Engineered for non-viral cell transfection, this mRNA-LNP product encodes a GA101-derived CAR targeting CD20. Dual co-stimulatory NKG2D and 2B4 motifs combined with CD3z signaling drive potent cytotoxic responses against B-cell lineages.

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CAR Information

  • Target Species
  • Human
  • Clone
  • GA101
  • Host
  • Mouse
  • CAR Construction
  • h(NKG2D-2B4-CD3z)
  • CAR Generation
  • Third
  • CAR Signaling Description
  • The h(NKG2D-2B4-CD3z) chimeric antigen receptor (CAR) is a specialized construct designed to enhance the anti-tumor activity of engineered immune cells, particularly Natural Killer (NK) cells or T cells, by combining multiple signaling domains. The design is a third-generation CAR incorporating an antigen-binding domain derived from the native NKG2D receptor and multiple intracellular signaling domains.

CAR mRNA Properties

  • Cap
  • Cap 1
  • PolyA
  • Segmented PolyA
  • Product Type
  • CAR mRNA LNP
  • Lipid Mixture
  • Utilizes a proprietary lipid platform composed of ionizable lipid, cholesterol, helper phospholipid, and PEG-lipid.
  • Concentration
  • Lot specific
  • Buffer
  • The formulation is buffered in either Phosphate-Buffered Saline (PBS) or a citrate buffer containing 10% sucrose to maintain lipid nanoparticle stability and prevent aggregation during freeze-thaw cycles.
  • Storage
  • Maintain at -80°C. Avoid repeated freeze-thaw cycles to preserve mRNA integrity and LNP size distribution.
  • Handling
  • Thaw slowly on ice prior to use. Avoid vigorous vortexing; gently invert the vial to mix in order to preserve LNP integrity.

mRNA Quality Control

  • OD260/280
  • PASS
  • Agarose Gel Mobility
  • PASS
  • IVT Vector Sequencing
  • PASS
  • IVT Vector Enzyme Digestion
  • PASS
  • mRNA Transfection Efficiency
  • PASS
  • CAR Expression after mRNA Transfection
  • PASS

Target Information

  • Target
  • CD20
  • Gene Name
  • MS4A1
  • Full Name
  • B-lymphocyte antigen CD20
  • Introduction
  • A B-cell surface protein involved in Ca2+ conductance and activation. It is a highly successful therapeutic target for non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukemia (CLL) via antibodies and CARs.
  • Related Diseases
  • Acute lymphoblastic leukemia (ALL)
  • Alternative Names
  • B-lymphocyte antigen CD20; MS4A1

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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