Exo-liposome Products
Product Details Publish Data FAQs Resources
Product Details
Creative Biolabs is dedicated to offering high-quality liposome products for clients. Our Exo-liposome is a cutting-edge product that mimics the lipid composition of exosomes with high precision. This enables the simulation and investigation of exosome functions, thereby driving significant advancements in your projects.
Exosomes are submicron vesicles with external membranes that are released by practically all cell types and contain vast amounts of nucleic acids and proteins. Their low immunogenicity and high biocompatibility make them suitable for safe and effective delivery of drugs. However, the use of these materials is limited due to their lack of large-scale production capacity and their composition, which changes depending on the kind of cell and physiological condition. Exosome mimetics can help to overcome these constraints.
With years of expertise in the field of liposomes, you can trust us to offer you the highest-quality products. If you have any questions or concerns about Exo-liposome, please contact us for more information.
Publish Data
One-Step Formation Method of Plasmid DNA-Loaded, Extracellular Vesicles-Mimicking Lipid Nanoparticles Based on Nucleic Acids Dilution-Induced Assembly
Author: Helgudóttir, Steinunn Sara, et al.
This study looked at the transfection efficacy and mechanism of exosome-mimicking lipid nanoparticles (ELNPs) in HepG2 cells. The researchers prepared ELNPs with lipid ratios of DOPC:DOPE:DOPS:SM:Chol = 18:7:13:17:45. Subsequently, They used chlorpromazine, MβCD, and EIPA in endocytosis inhibition experiments to investigate the mechanism behind the high transfection efficiency of ELNPs in HepG2 cells. The results showed that inhibiting clathrin-mediated endocytosis with chlorpromazine had no effect on transfection efficiency, but inhibiting lipid raft-mediated endocytosis with MβCD and macropinocytosis with EIPA significantly decreased transfection efficiency. This implies that the transfection process of ELNPs involves both lipid raft-mediated endocytosis and macropinocytosis. The work provides an approach to investigate natural exosome activity using exosome mimetics, without surface antigen interference.
Fig.1 Effects of endocytosis inhibition on the transfection efficiency of ELNPs.1,2
FAQs
What are the features of Exo-liposomes?
Exo-liposomes share a similar structure, composition, size, and Zeta potential as natural exosomes. Compared to plain liposomes, Exo-liposomes offer improved biocompatibility, bioactivity, and can mimic the functions of exosomes.
In which research areas are Exo-liposomes applicable?
Exo-liposomes are widely utilized in research areas such as gene delivery, drug delivery, immune modulation, and cell signaling transduction.
What is the stability of Exo-liposomes?
Exo-liposomes exhibit good stability when stored at 4°C and protected from light, maintaining their functionality and activity for up to six months.
Can Exo-liposomes be used for in vivo experiments?
Prior to conducting in vivo experiments with Exo-liposome, thorough safety assessments and rigorous experimental planning are necessary to ensure their safety and efficacy.
How to evaluate the impact of fluorescently labeled Exo-liposomes on cellular uptake efficiency?
You can assess the cellular uptake of fluorescently labeled Exo-liposome by flow cytometry and fluorescence microscopy.
Resources
References
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Okami, Kazuya, et al. "One-Step Formation Method of Plasmid DNA-Loaded, Extracellular Vesicles-Mimicking Lipid Nanoparticles Based on Nucleic Acids Dilution-Induced Assembly." Cells 13.14 (2024): 1183.
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under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not For Clinical Use
