Fluorescent Liposome Development Service

Q: What is the minimum particle size you can achieve for fluorescent liposomes?

We routinely achieve particle sizes down to 80–100 nm (mean diameter) with excellent polydispersity indices (PDI < 0.1), though the minimum size often depends on the lipid composition and encapsulation cargo.

Q: Can you develop fluorescent liposomes using client-supplied proprietary lipids?

Absolutely. Our Custom Synthesis team specializes in integrating client-supplied or proprietary lipids into novel fluorescent formulations while maintaining strict confidentiality protocols.

Q: What fluorescent dyes are most compatible with non-invasive in vivo imaging?

For in vivo studies, we recommend near-infrared (NIR) dyes, such as those from the DiR or Cy7 series. These fluorophores minimize tissue autofluorescence and maximize penetration depth for clearer biodistribution images.

Q: What is the typical shelf life of a Creative Biolabs fluorescent liposome formulation?

Shelf life varies based on the lipid composition and storage buffer, but our formulations are typically stable for 6 to 12 months when stored properly at 4°C, protected from light, and avoiding freezing.

Background Our Services Workflow Applications Why Choose Us Related Services & Products FAQs

The future of drug efficacy relies on precision delivery, and the cornerstone of successful liposome development is visualization. Accurately tracking the journey of a drug carrier—from administration to cellular uptake and cargo release—is non-negotiable for translational research. Creative Biolabs' Fluorescent Liposome Development Services provide the critical imaging tools necessary to achieve this verifiable performance. We offer custom-engineered carriers that deliver clarity and confidence to your preclinical studies, leveraging our two decades of specialized expertise in lipid-based drug delivery systems.

What are Fluorescent Liposomes?

Schematic diagram of fluorescent liposomes. (Creative Biolabs Original)

Fluorescent liposomes are nanoscale drug carriers where a highly specific fluorophore is strategically integrated. This dye acts as a precise tracer, enabling visualization using advanced techniques like confocal microscopy or non-invasive in vivo imaging systems. The versatility of fluorescent liposomes lies in the placement of the fluorophore: it can be incorporated into the aqueous core (for tracking internal payload) or inserted directly into the lipid bilayer (for monitoring membrane stability and fusion).

The Critical Role of Fluorescent Liposomes in Nanomedicine

The challenge in modern drug delivery lies not just in encapsulation, but in verifiable performance. Fluorescent labeling allows researchers to solve critical questions across the entire drug lifecycle:

Real-Time Tracking: Visualize biodistribution in vivo and monitor circulation time, informing the design of Long Circulating Liposome Development.
Cellular and Subcellular Fate: Determine precise cellular uptake mechanisms (e.g., endocytosis, fusion) and monitor the crucial step of endosomal escape.
Efficacy Mechanisms: Utilize advanced techniques to measure membrane stability, cargo release kinetics, and fusion events with unmatched accuracy.
Diagnostic Precision: Develop highly sensitive carriers for molecular imaging and non-enzymatic detection of disease biomarkers.

Comprehensive Fluorescent Liposome Development Services

Creative Biolabs' integrated service approach ensures your fluorescent liposome project moves rapidly from concept to clear visualization, reinforcing our mission to provide the highest-quality research tools. While we specialize in custom engineering, we also maintain a portfolio of popular fluorescent liposome products in stock to immediately support common research protocols, alongside our comprehensive custom development capabilities below.

Versatile Fluorophore Integration and Custom Synthesis

The integrity and placement of the fluorescent probe are essential for accurate results. Creative Biolabs offers flexibility across both lipophilic (membrane-inserted) and hydrophilic (aqueous-core) dye conjugation methods.

Comprehensive Dye Portfolio: We develop fluorescent liposomes using a diverse range of lipophilic and hydrophilic dyes, including the popular Di series (DiI, DiD, DiR), Cy series (Cy3, Cy5, Cy7), and classic hydrophilic probes like FITC and Rhodamine. This variety supports studies across the entire visible and near-infrared spectrum for single or multiplexed imaging.
Lipid Integration: Conjugation to specialized lipids, including cationic, ionizable, helper lipids, and PEGylated Lipids, crucial for liposome and long-circulating formulations.

Advanced Fluorescent Liposome Formulation & Optimization

Our service is centered on achieving optimal particle size, charge, and stability for your specific application.

Controlled Architectures: Expertise in preparing Unilamellar and Multilamellar Vesicles with controlled size extrusion (100nm–500nm) using proprietary methodologies.

Rigorous Formulation Analysis & Characterization

We provide complete, auditable data packages to demonstrate product quality and reliability.

Physicochemical Validation: Particle size (DLS), PDI, and zeta potential measurement.
Encapsulation & Purity: HPLC and mass spectrometry verification of lipid and fluorophore purity, and quantification of encapsulation efficiency.

Workflow

Contact & requirements One-to-one technical support Submit custom service form Project start Product delivery Optional Pharmacodynamic Study Analysis and Characterization

Step	Description	Starting Materials	Deliverables	Estimated Time
1	Project Scoping & Design	Client Target Criteria Initial Lipids Fluorophore Stock	Detailed Project Plan Lipid Composition Proposal QA/QC Plan	< 1 Week
2	Custom Synthesis & Formulation	Chosen Lipid Mixture Selected Fluorophore Solvents	Raw Fluorescent Liposome Batch Initial Characterization Data (PDI/Size)	2-4 Weeks
3	Optimization & Extrusion	Raw Liposomes Extrusion Membranes	Target Particle Size (e.g., 100nm ± 10nm)	2-4 Weeks
4	Rigorous Analysis & QC	Optimized Liposomes	Comprehensive Characterization Report (DLS, Zeta)	1-2 Weeks
5	Delivery	/	Product and COA	< 1 Week

Applications of Fluorescent Liposomes

Our services directly support high-impact research across therapeutic, diagnostic, and immunological fields:

Drug Delivery Optimization: Verify successful delivery to target tissues and confirm drug release kinetics in real-time, directly informing clinical translation.
In Vivo & Ex Vivo Imaging: Non-invasive imaging of biodistribution and clearance to calculate pharmacokinetic and pharmacodynamic (PK-PD) parameters.
Immunotherapy & Diagnostics: Tracking of carrier migration to lymphoid organs and monitoring cellular interactions, such as studying Macrophage Apoptosis or B-cell activation.
Neuroscience: Development of carriers engineered to cross the Blood-Brain Barrier (BBB), enabling precise visualization of delivery mechanisms in the CNS.

Why Choose Creative Biolabs for Fluorescent Liposomes Development?

Creative Biolabs is dedicated to accelerating your scientific discoveries. Our 20-year commitment to quality, innovation, and partnership ensures your project benefits from:

Unmatched Scientific Depth: Our team comprises veteran pharmaceutical scientists specialized in Nanotechnology and Drug carrier design.
Client-Centric Collaboration: We operate as a seamless extension of your R&D team, providing personalized support and flexible project management.
Commitment to Quality: All services adhere to strict QA/QC protocols, ensuring highly consistent, research-grade materials for sensitive assays.
Maximum Experimental Flexibility: Access a comprehensive portfolio of fluorescent liposomes featuring a variety of dyes (e.g., Di series, Cy series, FITC) optimized for either lipid membrane insertion or aqueous core encapsulation, ensuring the perfect match for your experimental setup.

Ready to gain high-resolution insight into your lipid-based drug delivery system? Then contact us immediately to obtain more details about fluorescent liposomes.