LNP Preparation Kits
Products Publish Data FAQs Resources
Product Details
As a leader in lipid-based delivery systems, Creative Biolabs has always been committed to developing and producing high-quality products. To provide convenience for researchers, we offer a series of kits designed specifically for the rapid preparation and evaluation of lipid nanoparticles (LNPs), without the need for specialized equipment. These kits are composed of different lyophilized lipids, such as ionizable lipids, helper lipids, PEGylated lipids, and cholesterol, among others. Depending on your needs, you can choose different kits to achieve the encapsulation of drugs, peptides, proteins, nucleic acids, and other materials.
Creative Biolabs' LNP preparation kit not only comes with reliable quality assurance but also provides professional after-sales service to help clients achieve better research outcomes in the field of lipid-based delivery systems. Interested clients are welcome to contact us to learn more about our LNP preparation kits!
Publish Data
Lipid Nanoparticles-Encapsulated YF4: A Potential Therapeutic Oral Peptide Delivery System for Hypertension Treatment
Author: Zhao, Shengnan, et al.
This study explores the impact of using LNP delivery of antihypertensive peptides on blood pressure-lowering (BPL) effects. Due to the susceptibility of antihypertensive peptides to degradation in the gastrointestinal tract, their short half-life and poor bioavailability are major concerns. Researchers prepared LNP loaded with Tyr-Gly-Leu-Phe (YF4) (YF4-LNPs) to address the inadequate therapeutic effects of the antihypertensive peptides. Subsequently, they investigated the BPL effect of free YF4 and YF4-LNPs on old male spontaneously hypertensive rats (SHRs). The results showed that the systolic blood pressure (SBP) of the free YF4 group decreased by 15.6 mmHg around 4 hours after administration, but returned to untreated levels around 12 hours. In contrast, the SBP of the YF4-LNPs group decreased by 43.5 mmHg around 2 hours after administration. Surprisingly, a significant BPL effect was observed even at 120 hours after administration, with a return to untreated levels not occurring until 144 hours after administration. This study indicates that LNP can extend the half-life of YF4 through controlled release, thereby enhancing its therapeutic efficacy.
Fig.1 Antihypertensive effects of free YF4 and YF4-LNPs in SHRs.1,2
FAQs
Do these kits require any specialized equipment to use?
Our kits typically provide most of the necessary materials and reagents, but you may need some basic laboratory equipment such as a microfluidic device and centrifuge. Please contact us for detailed instructions.
How to assess the quality of LNPs?
Techniques like dynamic light scattering (DLS) or transmission electron microscopy (TEM) can be used to assess the size, distribution, and shape of LNPs.
Can prepared LNPs be stored frozen?
Prepared LNPs should be used promptly. They can be stored at 4°C for up to 2 weeks but should not be frozen.
How to adjust the payload of LNPs?
The payload of LNPs can be controlled by adjusting the RNA or drug concentration, ratios, etc.
Resources
References
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Zhao, Shengnan, et al. "Lipid nanoparticles-encapsulated YF4: A potential therapeutic oral peptide delivery system for hypertension treatment." Frontiers in pharmacology 10 (2019): 102.
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under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not For Clinical Use
