Lipid Nanoparticle Products
Introduction of Lipid Nanoparticles (LNPs)
Lipid Nanoparticles (LNPs) are lipid vesicles with a uniform lipid core, prepared from ionizable/cationic lipids, helper/structural lipid, cholesterol, and PEGylated lipids, with a diameter of around 100 nm. Their unique structure and size give them special physical and chemical properties, making them effective for encapsulating and protecting nucleic acid drugs like mRNA, siRNA, and DNA. LNPs can adhere to negatively charged cell membranes and are taken up by cells via endocytosis, forming endosomes. These mature endosomes fuse with lysosomes, where the acidic environment triggers the protonation of ionizable lipids, giving them a positive charge. This allows LNPs to escape the endosomes and release their nucleic acid cargo into the cytoplasm.
Advantages and Applications of Lipid Nanoparticles
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High Payload Capacity and Delivery Efficiency: LNPs can effectively encapsulate and protect long nucleic acid drugs, preventing their degradation and premature release during delivery.
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Surface Modification: By conjugating LNPs with antibodies, peptides, and other ligands, targeted delivery to specific tissues, organs, and cells can be achieved.
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Nucleic Acid Delivery: LNPs can carry various nucleic acids, including mRNA, siRNA, circular RNA, antisense oligonucleotides, and DNA.
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Gene Editing: LNPs can be loaded with Cas9 mRNA and sgRNA to prepare CRISPR-LNPs for gene editing applications.
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CAR-T Therapy: LNPs can facilitate in vivo CAR T cell production by delivering CAR mRNA to lymphocytes, offering efficient delivery, low immunogenicity, and reduced risk of insertional mutagenesis.
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SORT Technology: Based on traditional four-component lipids, introducing a fifth lipid component enables targeted delivery to specific tissues, organs, and cells.
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SORT Molecule
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Charge
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Lipid
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Target
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Cationic Lipid
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Positive
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50% DOTAP
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Lung
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Anionic Lipid
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Negative
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10-40% 18PA
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Spleen
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Creative Biolabs' Lipid Nanoparticle Products
Ionizable/cationic lipids are key to LNP development. We offer off-the-shelf LNPs with diverse ionizable lipids, customizable with capped/modified nucleic acids.
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LNPs for delivering various nucleic acids (reporter genes, gene-editing, vaccines/antigens, etc.).
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Empty LNPs as controls to find the optimal LNP formula.
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mRNA with/without caps and modifications to boost expression and reduce immunogenicity.
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Suitable for both cellular and animal experiments.
For specific needs, explore our LNP development services or email us at info@creative-biolabs.com for details.
Please refer to our detailed list of LNP products:
Quality Control
Creative Biolabs guarantees the supply of LNPs with uniform particle size and exceptional stability, ensuring dependable and effective genetic material delivery for a wide range of research applications.
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Item
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Method
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Specification
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Particle size
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Dynamic light scattering
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Uniform size distribution (typically around 100nm)
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Poly Dispersity Index (PDI)
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Dynamic light scattering
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< 0.3
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Zeta Potential
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Dynamic light scattering
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Varies with different lipid compositions
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Encapsulation Efficiency
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RiboGreen
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>85%
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Concentration
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RiboGreen
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Customizable concentration
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*For more testing items, please contact us.
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Case Study: SM102 LNP-Fluc mRNA (m1Ψ) ( CAT#: LDLY-0325-LD501)
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Size (Z-average): The average hydrodynamic diameter of nanoparticles, reflecting the overall particle size in solution.
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PDI: A measure of nanoparticle size uniformity and distribution width.
FAQs
What is the significance of Zeta Potential in LNPs?
Zeta Potential indicates the surface charge of LNPs, and a higher absolute value generally suggests superior stability and cellular uptake.
I need assistance designing my LNP formulation. Do you offer consulting services?
Yes, our team of specialists can provide consultation for LNP formulation and optimization.
What is the shelf life of Creative Biolabs' LNP products?
Our LNP products have an expected shelf life of 6-12 months under proper storage conditions.
What is the delivery efficiency of Creative Biolabs' LNPs for different types of nucleic acids?
Our LNPs have high delivery efficiency for various nucleic acids, including mRNA, siRNA, DNA, etc.
What is the estimated delivery period for custom LNP orders?
The estimated delivery period for custom LNPs is influenced by the formulation's complexity and our production planning. We make every effort to finish orders within 2-6 weeks.
Can I provide a reference for the LNP formulation I need?
Definitely! If you have a specific formulation from a published study, share the details with us, and we will work to replicate it for your project.
Can I get LNPs with specific fluorescence labels for tracking purposes?
Yes, we can incorporate fluorescence labels into LNPs to facilitate tracking and imaging in your research experiments.
I need to scale up my LNP production for a larger study. Can you support this?
Yes, we offer scalable production solutions to meet the demands of larger studies while maintaining product quality.
Resources
For Research Use Only. Not For Clinical Use
