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Quantitative Human DMBT1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX875)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human DMBT1 (glycosylated) sandwich ELISA kit is designed to detect human deleted in malignant brain tumors 1 (DMBT1) levels. DMBT1 is a secreted glycoprotein that plays a role in mucosal defense. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.068 ng/mL, which can accurately detect low concentrations of DMBT1 in the sample.
Target DMBT1
N-Glycosylation Site 566, 737, 1712, 1745, 1818, 1832, 1842, 1889, 1998, 2120, 2188, 2233, 2240, 2256
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.068 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Deleted in malignant brain tumors 1
Alternate Names DMBT1; Deleted in malignant brain tumors 1; GP340; SAG; Glycoprotein 340; Salivary agglutinin
Uniprot No. Q9UGM3
Application The quantitative human DMBT1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of DMBT1 in biological samples. This kit is applicable in studies examining mucosal immunity and tumor suppression. It is also relevant to studies investigating the role of DMBT1 in protecting mucosal surfaces and its potential involvement in preventing tumor development.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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