Chitosan Analysis Service

Introduction and Main Categories of Chitosan

Chitosan is a natural high molecular weight polysaccharide obtained by deacetylation of chitin. Chitin is widely found in the shells of crustaceans (such as shrimps and crabs), the cell walls of fungi, and other parts, and it is the second-largest natural polysaccharide after cellulose. Chitosan has garnered significant interest because of its biocompatibility, degradability, and versatility. With the continuous expansion of application areas, the quality and performance requirements of chitosan products are also getting higher and higher. Providing professional chitosan analysis services not only aids in ensuring product quality and satisfying clients but also fosters technological innovation and supports the sustainable development of the industry.

Chitosan is classified according to different criteria, which help researchers and application developers select the appropriate type of chitosan according to different physicochemical properties, uses, and performance.

Professional Chitosan Analysis Service at Creative Biolabs, Promotes Sustainable Development of the Industry.

Creative Biolabs has a professional technical team with rich experience in Monosaccharide, Oligosaccharide, Polysaccharide, and other Carbohydrate Analysis projects. We use advanced analysis techniques to ensure the accurate determination of various physical and chemical indicators of chitosan. The following is the chitosan analysis content we provide.

• Degree of deacetylation analysis

The degree of deacetylation is an important parameter of chitosan, reflecting the degree of removal of acetyl groups in chitosan, which directly affects its solubility and biological activity. We use techniques such as nuclear magnetic resonance spectroscopy (NMR) and infrared spectroscopy (FTIR) to determine the degree of deacetylation of chitosan.

• Molecular weight analysis

Molecular weight affects the physical and biological properties of chitosan. We combine gel permeation chromatography (GPC) and size exclusion chromatography (SEC) with multi-angle laser light scattering (MALLS) to determine the molecular weight and distribution of chitosan.

• Component analysis

We use precise analysis methods to detect the content of various components in chitosan, such as chitobiose, chitotriose, chitotetraose, chitopentaose, chitohexaose, etc., to provide clients with accurate component information and help clients understand the quality and characteristics of chitosan.

• Structural characterization

We use spectral analysis and other technical means to characterize the molecular structure of chitosan in detail, including analyzing the type of its chemical bonds, the distribution of functional groups, etc., to provide a theoretical basis for its application in different fields.

In addition, Creative Biolabs also provides chitosan physical property measurement services to help clients better understand the physical properties of chitosan. We conduct testing in strict accordance with international standards and specifications to ensure high-quality chitosan analysis results. Moreover, we also provide personalized technical solutions according to the specific needs of clients to help them better utilize the characteristics of chitosan to develop new products or improve existing products. Please contact us if you would like to learn more about our chitosan analysis services or have any questions.

Published Data

The research explores the application of spectroscopic techniques in characterizing the structure of chitin and chitosan. A key focus is on detailing various mass spectrometry (MS) approaches employed in chitosan analysis. Moreover, the study emphasizes the role of NMR spectroscopy in determining crucial structural attributes of chitosan. Specifically, NMR is utilized to ascertain the degree of N-acetylation (DA), which indicates the proportion of deacetylated units in the chitosan polymer, and the pattern of acetylation (PA), which describes the distribution of acetyl groups along the polymer chain. The research underscores the importance of these parameters, DA and PA, as they significantly influence the overall properties and functionalities of both chitin and chitosan. The selection of appropriate analytical methods is crucial for accurately characterizing these biopolymers.

Fig.1 Schematic diagram of the structures of chitin and chitosan.¹

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Reference

1. Kumirska, Jolanta, et al. "Application of spectroscopic methods for structural analysis of chitin and chitosan." Marine Drugs 8.5 (2010): 1567-1636. Distributed under Open Access license CC BY 3.0, without modification.

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