Anti-Poly-Sialic Acid Antibody Development Service
Overview
Polysialic acid is a unique glycan composed of α-linked sialic acid residues, classified primarily into α2,8-linked and α2,9-linked forms. These chains differ in linkage pattern and degree of polymerization, resulting in diverse biological functions. While α2,8-linked polysialic acid is commonly associated with neural cell adhesion molecules and developmental processes, α2,9-linked structures are often present in bacterial capsules. In human health, re-expression of polysialic acid has been observed in a variety of tumors, where it promotes cancer cell migration, immune evasion, and metastatic potential. This makes polysialic acid a critical biomarker and therapeutic target, underscoring the importance of developing high-quality, selective antibodies against it.
Anti-poly-sialic acid antibody development service at Creative Biolabs helps you accelerate discovery and achieve robust, DP-sensitive detection through advanced immunogen design, recombinant antibody engineering, and validated screening platforms.
Our strategies for anti-polysialic acid antibody development are built on three pillars:
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Precision immunogen design using well-characterized polysialic acid fractions.
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Recombinant antibody engineering to enhance affinity, specificity, and stability while reducing cross-reactivity.
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Multi-platform screening and validation, including ELISA, flow cytometry, BLI, and enzymatic controls (Endo-N) to confirm specificity.
By leveraging our expertise, clients gain access to precise, chain-length–sensitive antibodies with demonstrated performance in complex biological contexts.
Learn How We Can Accelerate Your Research – Request a Consultation Today.
What We Can Offer
Custom Conjugation Service
We provide conjugation of polysialic acid to a variety of carrier proteins or solid-phase supports, enabling efficient immunization, library screening, and assay development tailored to client requirements.
Custom Antibody Development
We design and generate monoclonal or polyclonal antibodies specifically tailored to α-linked polysialic acid epitopes, ensuring optimal specificity and reproducibility.
Multipurpose Validation
Every antibody is validated across multiple applications such as IHC, IF, WB, and flow cytometry, allowing seamless integration into your experimental workflow.
Comprehensive Analytical Support
We supply clients with detailed characterization data, including chain-length recognition profiles, binding kinetics, and cross-reactivity analysis, ensuring each antibody performs reliably in complex biological samples.
Workflow
01Project consultation
In-depth discussion of project goals, antibody format preferences, and intended applications.
02Immunogen preparation
Careful selection of defined polysialic acid fractions and conjugation to protein carriers (KLH, BSA, OVA) to boost immunogenicity and guide antibody specificity.
03Antibody generation
Application of hybridoma or phage display platforms to produce diverse antibody candidates aligned with project needs.
04Screening & validation
High-throughput evaluation of candidate antibodies using multiple assay formats to verify binding specificity.
05Affinity optimization
Engineering and maturation strategies applied to improve binding strength, stability, and functional performance.
06Final characterization
Rigorous quality control including cross-reactivity profiling, stability assessment, and validation across multiple assay formats.
Highlights
Expertise in Glycobiology
With extensive experience in polysialic acid biology, Creative Biolabs offers an unmatched understanding of structure-function relationships critical for precise antibody development.
Advanced Engineering Platforms
Our recombinant antibody technologies enable affinity maturation, isotype switching, and humanization to meet both research and translational needs.
Comprehensive Quality Control
We incorporate enzymatic digestion, DP profiling, and orthogonal analytical assays to confirm antibody specificity and reliability.
Flexible Project Models
From discovery research to clinical-stage development, we tailor project scope and deliverables to align with each client’s goals.
Publication
Polysialic acid plays a dynamic role in adaptive immunity, where its expression is closely linked with the activity of polysialyltransferases. In lymphocytes and other immune cells, this glycan acts as a structural and functional regulator, modulating cell–cell interactions and fine-tuning immune responses. Expression patterns reveal that polysialic acid is not uniformly distributed but rather selectively upregulated in activated subsets, highlighting its role in immune activation and differentiation. The associated polysialyltransferases contribute to shaping polysialic acid chain length and density, thereby influencing receptor accessibility and signaling thresholds. This finely controlled system underscores the importance of glycan remodeling in immune cell plasticity and offers valuable insight into how polysialic acid supports both immune regulation and potential therapeutic targeting.
Fig.1 Expression of polysialic acid and polysialyltransferases in adaptive immune cells.1
Customer Reviews
Improved tumor detection
“Using Creative Biolabs’ anti-polysialic acid antibody development service in our oncology pipeline significantly enhanced our ability to detect polysialylated NCAM in breast cancer biopsies, providing clearer diagnostic insights.” [Researcher, Dr. J***]
Reliable immune profiling
“Incorporating Creative Biolabs’ custom polysialic acid antibody in our immunology project allowed accurate monitoring of activated immune cells, improving our data reproducibility compared to commercial clones.” [Scientist, Dr. L***]
Enhanced assay sensitivity
“Our neuroscience lab benefited from Creative Biolabs’ engineered antibody, which offered higher affinity for long-chain polysialic acid, enabling us to quantify subtle differences in brain tissue samples with confidence.” [Principal Investigator, Prof. M***]
FAQs
What applications can anti-polysialic acid antibodies be used for?
They are widely applied in oncology, neuroscience, and immunology to detect, quantify, or target polysialylated proteins and cells.
What formats are available for developed antibodies?
We offer monoclonal, recombinant, chimeric, and humanized formats suitable for both research and preclinical use.
How do Creative Biolabs’ services compare to using commercial clones?
Commercial clones are excellent for baseline studies, but our service offers customized, affinity-optimized antibodies with improved performance for your specific research needs.
Extended Services
To complement our anti-polysialic acid antibody development, Creative Biolabs also provides:
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Glycoengineering services for customized glycan-based immunogens.
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Anti-glycan antibody development targeting diverse glycans beyond polysialic acid.
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Antibody humanization and affinity maturation for translational projects.
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Diagnostic assay development support for biomarker validation and clinical applications.
Creative Biolabs’ anti-polysialic acid antibody development service offers precision solutions from immunogen design to final antibody validation. Our service enables the detection of polysialic acid in tumor microenvironments, monitoring of immune responses, and exploration of therapeutic pathways. Partnering with Creative Biolabs ensures minimized risk of cross-reactivity, robust assay performance, and reproducible results. To learn more and discuss your project in detail, Contact Our Team today.
Reference
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Villanueva-Cabello, Tania M et al. “Polysialic Acid in the Immune System.” Frontiers in immunology vol. 12 823637. 11 Feb. 2022. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fimmu.2021.823637
For Research Use Only.
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