Mannoligosaccharide Analysis Service

Introduction of Mannoligosaccharides (MOS)

It's interesting how certain things in science start to gain real traction, such as mannoligosaccharides (MOS). You hear about them more and more these days, especially when folks are talking about the latest in biotech and food science. MOS is a type of sugar-based molecule, a chain made up of mannose units linked together. There are these specific ways they connect, called α-1,4 or β-1,4 glycosidic bonds. What's fascinating is that you can find these things naturally in so many places, including plants we see, the yeast used in baking, and even in the world of bacteria. Because of this potential, in areas from making our food better to maybe even new kinds of medicine, really getting a good understanding of what MOS is and how much is present seems like a pretty big deal for researchers making things.

Professional MOS Full-Process Analysis at Creative Biolabs

As an expert in the field of Carbohydrates, Creative Biolabs has a scientific research team with many years of experience in Oligosaccharide Analysis and provides you with professional MOS full-process analysis services, covering mannose, mannobiose, mannotriose, mannotetrose, mannohexaose, and other components. The following is our conventional MOS analysis process, which helps you better understand and choose our services.

• Sample preparation

We use appropriate extraction methods for different sample matrices, such as hot water extraction, dilute acid extraction, or enzymatic hydrolysis, to release MOS from complex matrices. We strictly control the temperature, time, and pH value during the extraction process to prevent the degradation or isomerization of sugars.

• Sample purification

The extracted samples usually contain a variety of impurities and need to be further purified. We use solid phase extraction (SPE), ultrafiltration, and precipitation to remove impurities from the samples. Moreover, we select appropriate media and buffers to ensure the stability and recovery rate of MOS to improve the accuracy of analysis.

• Sample separation
  • Liquid chromatography (HPLC): To improve the sensitivity and specificity of detection, derivatization treatment is often necessary. We use HPLC to separate and quantify the derivatized MOS. According to the properties of MOS, we select the appropriate chromatographic column and optimize the chromatographic conditions to obtain the best separation effect. Commonly used detectors include ultraviolet detectors (UV) or fluorescence detectors (FLD).
  • Ion chromatography (IC): IC can effectively separate and detect MOS without derivatization.
  • Mass spectrometry (MS): MS combined with HPLC, obtains the molecular weight and fragment ion of MOS, thereby inferring its structural composition. Electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are commonly used analysis methods.

• Data analysis

We carefully analyze and compare the obtained data and use a variety of analysis methods to comprehensively evaluate the composition, structure, and content of MOS.

Advantages

• Leading technology: Equipped with high-end instruments such as HPLC and MS to support complex matrix analysis.
• Experienced: Analytical chemistry experts with rich experience have served more than 2000+ clients, covering multiple fields such as food, agriculture, and environmental protection.
• Customized solutions: Design exclusive detection processes according to sample characteristics and provide data analysis and interpretation.
• Full-cycle service support: Efficient service processes help customers speed up project progress.

Creative Biolabs has provided reliable project analysis for Monosaccharides, oligosaccharides, and Polysaccharides to many well-known companies and scientific research institutions, assisting them in completing product development and quality improvement, and has won unanimous praise from clients. We provide clients with efficient, accurate, and reliable carbohydrate analysis solutions. Please contact us if you need more information about MOS analysis services or have any technical questions. We look forward to working with you to promote the development of the industry jointly.

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