What Is Trimethylamine N-oxide?
Trimethylamine N-oxide (TMAO) is a small, organic compound increasingly recognized as a significant metabolite implicated in various human diseases, particularly cardiovascular disease (CVD) and metabolic disorders. Formed primarily in the liver from the oxidation of trimethylamine (TMA), which is produced by gut microbiota from dietary precursors like choline and L-carnitine, TMAO acts as a critical link in the gut-heart axis. Increased levels of plasma TMAO are significantly linked to a higher risk of atherosclerosis, myocardial infarction, stroke, and chronic kidney disease, typically influenced by inflammatory pathways. Accurate quantification of TMAO and its precursors is therefore essential for biomarker discovery, disease risk assessment, and Metabolism Research.
Advancing Health Insights: TMAO Quantitative Analysis Service at Creative Biolabs
Are you currently facing challenges in accurately quantifying complex metabolites, understanding their role in disease, or developing robust diagnostic assays? Our TMAO quantitative analysis service helps you gain precise insights into TMAO and related compounds, accelerating your research and development through advanced mass spectrometry (MS) platforms and expert bioanalytical methodologies. Our service delivers highly accurate and reliable quantification of TMAO and its precursors, providing critical data for understanding disease mechanisms, validating biomarkers, and assessing therapeutic interventions. We offer customized solutions designed to meet your unique research or clinical requirements, guaranteeing that you obtain practical insights. Creative Biolabs' TMAO quantitative analysis service follows a meticulously designed workflow to ensure precision, reproducibility, and timely delivery of results. This process is optimized for clarity and efficiency.
Types of TMAO Quantitative Analysis Service
Creative Biolabs provides a versatile range of TMAO quantitative analysis services, meticulously designed to cater to diverse research and applications. We provide precise quantification of TMAO and its key related compounds, including:
1
Carnitine: It is a quaternary ammonium compound that plays a role in fatty acid metabolism and acts as a precursor to TMA.
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Choline: An essential nutrient and precursor to TMA, found in various dietary sources.
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Betaine: A derivative of choline, also a precursor in the TMA/TMAO pathway.
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Creatinine: A waste product of muscle metabolism, often used as a normalization factor in urine analysis.
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TMA: The immediate precursor to TMAO, produced by gut microbes.
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TMAO: The oxidized product of TMA, a key circulating metabolite.
Creative Biolabs is your trusted partner for precise and comprehensive TMAO quantitative analysis services. With our advanced platforms, expert team, and commitment to scientific excellence, we also provide other compound analyses related to metabolic research, including Organic Acid, Catechin, Anthocyanin, Alkaloid, Flavonoid, Neurotransmitter, etc. We provide the critical insights needed to drive your research forward, from understanding disease mechanisms to developing novel therapeutics. Please contact us to discuss your specific project requirements and discover how Creative Biolabs can empower your scientific endeavors.
Published Data
The authors used ultra-performance liquid chromatography-multiple reaction monitoring mass spectrometry (UPLC-MRM-MS) to accurately measure the levels of TMA, its oxidized form trimethylamine-N-oxide, and several related compounds in biological samples. The methodology involves a meticulous preparation process, including methanol extraction and a chemical modification step using iodoacetonitrile (IACN). The study rigorously confirmed the method's reliability, demonstrating excellent linearity across a range of concentrations, high precision with low variability (2-8%), and strong recovery rates (exceeding 94%) for all targeted metabolites, which also included choline, betaine, L-carnitine, acetyl-L-carnitine, and propionyl-L-carnitine.
Fig.1 UPLC-MRM-MS quantitative determination of TMA, TMAO, and related metabolites.1
FAQs
Q1: What types of samples can you analyze for TMAO and its precursors?
A1: We are equipped to analyze various biological samples, including serum, plasma, and urine. Additionally, we offer specialized analysis for dietary samples such as fish oils and other food matrices. Our versatile methods ensure accurate quantification regardless of your sample type.
Q2: Can you help with interpreting the TMAO data in the context of my specific research?
A2: Absolutely! While our primary deliverable is a comprehensive analytical report with quantified concentrations, we also offer optional consultation services. Our team of expert scientists can help you understand the results and incorporate them into your wider research goals, offering valuable insights.
Q3: What is the core technology for detecting TMAO? What are the advantages of this method?
A3: We use LC-MS/MS technology for TMAO quantitative analysis. This method uses HPLC to separate TMAO and other metabolites (such as choline and betaine) in the sample and then detects the characteristic ion pairs of each target compound by tandem mass spectrometry in MRM mode. This method has the advantages of high sensitivity, high specificity, and a wide dynamic range.
Customer Review
Exceptional Precision
"Using Creative Biolabs's TMAO quantitative analysis service in our research has significantly improved the reliability of our metabolic profiling, providing highly accurate and consistent data that was crucial for our publication on gut-heart axis interactions." - Mr. Y. Hal***l
Streamlined Workflow
"The comprehensive analytical report and the clear communication from Creative Biolabs's team facilitated our understanding of TMAO dynamics in our dietary intervention study. Their service is incredibly efficient, allowing us to focus on data interpretation rather than method development." - Dr. Z. You***g
Reference
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Hefni, Mohammed E., and Cornelia M. Witthöft. "Development and Application of a UPLC-MRM-MS Method for Quantifying Trimethylamine, Trimethylamine-N-Oxide, and Related Metabolites in Individuals with and Without Metabolic Syndrome." Separations 12.2 (2025): 53. DOI: 10.3390/separations12020053. Distributed under an Open Access license CC BY 4.0, without modification.
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