Glycans are monosaccharides connected by glycosidic bonds, and glycoproteins are proteins containing one or more sugar chains. They play irreplaceable roles in many biological processes. Due to their structural complexity and high heterogeneity, studying these molecules is challenging, and labeling technology effectively helps scientists track, quantify, and analyze the functions and dynamic changes of glycans and glycoproteins. Based on this, Creative Biolabs has developed a series of high-quality glycan and glycoprotein labeling kits, which are usually combined with specific chemical reagents and reaction conditions to efficiently and accurately label glycans or glycoproteins in samples to explore and understand the complex roles of glycans and glycoproteins in biological systems.

Glycan Labeling Kit

Glycan labeling kits are mainly used to specifically label free glycans, usually by chemically binding fluorescent dyes or other probes to the free-reducing ends of glycans. These kits are widely used in biomedical research, especially in glycobiology, metabolic analysis, and disease diagnosis. Labeled glycans are analyzed by mass spectrometry (MS), and other techniques to identify their structure and functions. Common glycan labeling reagents include 2-aminobenzoic acid (2-AA), 2-aminobenzamide acid (2-AB), procainamide (PROC), etc.

Glycoprotein Labeling Kit

Glycoprotein labeling kits are specifically used to label sugar chains in glycoproteins. These kits combine labeling reagents with sugar chains in glycoproteins through chemical reactions. In this process, the reagents specifically recognize and bind to certain types of sugar molecules to form stable complexes. In addition, we also provide a variety of protein labeling kits. These labeled proteins are used in various downstream applications, such as Western blot, ELISA, MS, etc.

General detection process of labeling kit. (Creative Biolabs Original)

Diverse Labeling Reagents

Fluorescent dyes: Such as Cy3, Cy5, fluorescein isothiocyanate (FITC), etc. These dyes are combined with glycans or proteins and detected by fluorescence microscopy or flow cytometry for quantitative analysis and imaging.
Biotin: Biotin labeling is immunodetected by antibody binding and is commonly used in experiments such as Western blot and ELISA.
Chemical crosslinkers: Such as N-hydroxysuccinimide (NHS), these reagents are often used to promote the labeling of glycans or proteins in chemical reactions, improving the efficiency and selectivity of labeling.
Enzymes: Such as sialyltransferase (ST6Gal), increase the stability and specificity of labeling.
Radioisotopes: They bind to glycans or proteins under specific conditions, thus being used for tracking and quantitative analysis.

(Creative Biolabs Authorized)

Glycan and glycoprotein labeling kits are widely used in basic research, including the discovery of disease biomarkers, drug development, and biotechnology applications. Moreover, these kits are also combined with various analytical techniques to achieve a comprehensive analysis of glycans and glycoproteins in complex biological samples. Please feel free to contact us if you would like to purchase any labeling kit products.

For Research Use Only.

Glycan and Glycoprotein Labeling Kits