Quantitative Sialic Acid Analysis Service
Quantitative Analysis of Sialic Acid at Creative Biolabs
As a component of oligosaccharide units in glycoproteins, glycolipids, and mucins, sialic acid is widely present in nature, among which N-acetylneuraminic acid and N-glycolylneuraminic acid are the two most important sialic acids. The content and structural changes of sialic acid in glycoproteins are related to various diseases, such as cancer, tumors, neurodegenerative diseases, etc. Creative Biolabs has an advanced analytical technology platform, we provide professional Glycoprotein Analysis and quantification, including sialic acid analysis, monosaccharide analysis, and glycosaminoglycan analysis services.
Our analytical experts have many years of rich experience in sialic acid analysis, and use a variety of technologies and methods to provide high-quality sialic acid quantitative analysis services.
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Colorimetry
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Pretreatment: We perform necessary pretreatment steps on glycoprotein samples to remove impurities or other interferences.
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Spiking: We add an internal standard substance of known concentration to the sample as a reference substance.
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Hydrolysis: We perform hydrolysis on glycoprotein samples, breaking them down into simple sugars and sialic acid.
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Oxidation: We react the hydrolyzed sample with sodium iodate to oxidize sialic acid to dialdehyde under low pH conditions.
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Reaction: We react the oxidized sample with resorcinol to form a purple complex.
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Determination: Finally, we measure the absorbance or fluorescence intensity of the generated purple complex through the colorimetric method.
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Calculate concentration: We use sialic acid standards of different concentrations to establish a standard curve and then calculate the concentration of sialic acid in the sample according to the results of the standard curve and the absorbance or fluorescence intensity of the sample.
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Enzymatic method
We add sialyl hydrolase (such as sialidase) to the glycoprotein sample and optimize the reaction temperature, pH, and other conditions of the enzyme to release sialic acid in the glycoprotein. We then use chromatographic techniques or other analytical methods to separate the hydrolyzate and analyze it quantitatively or qualitatively. The enzymatic method is a method with high specificity and relatively simple operation, which is suitable for studying the content and structure of sialic acid in glycoproteins.
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Chromatography-mass spectrometry
We extract and purify glycoprotein samples, achieve separation of sialic acid by optimizing chromatographic conditions, and perform mass spectrometry analysis. The chromatography-mass spectrometry method combines the separation power of high-performance liquid chromatography (HPLC) with the sensitivity and specificity of mass spectrometry, which is used to accurately determine the sialic acid content in glycoproteins and provide critical information for biomedical research.
We use a fluorescein labeling agent to react with sialic acid to generate a fluorescent product, and then use a fluorescence spectrometer or fluorescence plate reader to measure the fluorescence intensity in the sample to calculate the sialic acid content. The fluorescence method has high sensitivity, high selectivity, and a wide linear range, which is suitable for analyzing the sialic acid content in glycoproteins.
Publication
Technology: Fluorescent high-performance liquid chromatography (HPLC) analysis
Books: SialoGlyco Chemistry and Biology II: Tools and Techniques to Identify and Capture Sialoglycans
Published: 2015
Results: The authors described a variety of analytical methods for sialic acid detection, among which fluorescence HPLC analysis was the most sensitive method for quantitative detection of sialic acid. The authors first acidolyzed the sialic acid glycoconjugates, then fluorescently labeled the released sialic acid with 1,2-diamino-4,5-methylenedioxybenzene (DMB), and finally the sialic acid was separated and quantified on HPLC. The results showed that this assay can rapidly separate nine different sialic acid derivatives.
Fig.1 Fluorescent HPLC analysis results of sialic acid derivatives.1
Advantages
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We have advanced detection equipment and professional analysis software, which can accurately and quickly analyze sialic acid.
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We have standardized experimental procedures and quality control procedures to ensure the stability and repeatability of the sialic acid analysis process.
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We provide fast and efficient analysis services, providing timely results to our clients. Moreover, we also provide personalized sialic acid analysis solutions.
Application
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Disease monitoring: Changes in sialic acid levels are associated with a variety of diseases, such as liver disease, cancer, inherited metabolic disorders, etc. Sialic acid analysis helps diagnose and monitor disease progression.
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Drug development: During drug development, sialic acid analysis is used to evaluate the impact of new drugs on metabolic pathways, verify drug efficacy, and understand drug interactions.
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Food: Sialic acid analysis is used to detect the sialic acid content in food, helping to evaluate the freshness, quality, and authenticity of food and ensuring food safety.
Creative Biolabs has rich experience in glycoprotein analysis and professional teams, which enjoys a good reputation in the field of bioanalysis. We are committed to providing clients with high-level sialic acid analysis services. Please contact us if you are interested in our analysis service.
Reference
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Kitajima, Ken, Nissi Varki, and Chihiro Sato. "Advanced technologies in sialic acid and sialoglycoconjugate analysis." SialoGlyco Chemistry and Biology II: Tools and Techniques to Identify and Capture Sialoglycans (2015): 75-103.
For Research Use Only.
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