Tumor Cell Line Glycoprofiling Service

Comprehensive Glycoprofiling of Tumor Cell Line at Creative Biolabs

Creative Biolabs has rich experience and an Advanced Analysis Technology platform, providing clients with a wide range of glycoprofiling analysis services, including antibodies, serum, plasma, tumor tissue, tumor cell lines, urine, and other biological samples. Glycoprofiling analysis of tumor cell lines helps reveal the metabolic characteristics and biological behavior of tumor cells, providing important information for tumor diagnosis and disease mechanism research. Glycoprofiling of tumor cell lines mainly involves the following steps:

• Culture of tumor cell lines: We culture tumor cell lines under sterile conditions to ensure good cell growth.
• Cell collection: We collect tumor cells using appropriate methods (such as trypsinization).
• Washing of cells: We wash the cells with a buffer such as saline or PBS to remove impurities produced in the culture medium and during cell culture.
• Cell lysis: We use an appropriate cell lysis buffer to lyse the cells and release the internal components.
• Precipitation of proteins: We centrifuge the cell lysate and collect the supernatant. Then add solvents such as ethanol or acetone to precipitate the protein to remove proteins that will affect subsequent analysis.
• Extraction of sugars: We use a mixed solvent system such as chloroform-methanol to extract sugars and separate the organic layer and aqueous layer. The organic layer is then transferred to a new tube and the solvent is removed by concentration and drying.
• Derivatization: We perform derivatization on sugar samples, such as methylation, etc. to facilitate subsequent analysis.
• Glycoprofiling of tumor cell line: We dissolve derivatized sugar samples in appropriate solvents to facilitate further chromatography or mass spectrometry analysis. For prepared tumor cell line samples, we use a variety of techniques to perform glycoprofiling:
  • Chromatography technology: We inject the sample into the chromatography system and separate the different sugar components through column separation technology. Subsequently, appropriate detectors are used for detection and quantification, resulting in a glycan profile.
  • Mass spectrometry: We perform glycan analysis through a variety of mass spectrometry techniques, which are suitable for analyzing sugars in complex mixtures and have high sensitivity and accuracy.
  • Nuclear magnetic resonance (NMR): It is used to determine the structure of sugars, identify isomers, and perform quantitative analysis.
  • Fluorescent labeling technology: We label sugars with specific fluorescent labeling agents and perform qualitative and quantitative analysis through fluorescence detection technology.

Fig.1 Detailed process for glycoprofiling of tumor cell lines.

Publication

Technology: Enzyme-linked lectin assay (ELLA)

Journal: Cancer Medicine

IF: 4.000

Published: 2022

Results: Cisplatin is a chemotherapeutic drug for the treatment of testicular cancer (TC). The authors analyzed the glycan changes of four different TC cell lines and their cisplatin-resistant sublines through the ELLA protocol. They chose the TC biomarker human chorionic gonadotropin (hCG) as the target for glycoprofiling analysis. hCG is present in the cytoplasm of cell lysates, and the authors immobilized the antibody on an ELISA plate and incubated it with the cytoplasm of the cell lysates and a variety of lectins. Results showed that dolichos biflorus agglutinin (DBA) differentiated between resistant (shown by red bars) and sensitive TC cell lines (shown by green bars).

Fig.2 Results of lectin binding to hCG in the cytoplasm of various cell lines.^{1, 2}

Advantages

• Professional leadership: We have a leading position in the field of glycoprofiling analysis of tumor cell lines and have accumulated rich experience and technical advantages.
• Custom service: We provide personalized glycoprofiling solutions according to client needs to meet different project needs.
• High-quality data: We provide high-quality, reliable glycoprofiling data and deliver reports promptly.

Application

• Glycoprofiling of tumor cell lines helps evaluate the effects of different drugs on cell lines and guides drug screening and development.
• Glycoprofiling of tumor cell lines helps predict patients' response to immunotherapy, thereby designing personalized treatment plans for patients to improve treatment effects and reduce patient adverse reactions.
• Exploring the glycoprotein modification patterns in tumor cell lines can reveal the role of protein post-translational modifications in tumor development, which helps to deeply understand and study the mechanisms of the disease.

Creative Biolabs has experienced scientists and advanced Glycoprotein Analysis technology. We pay attention to the latest trends in the industry and constantly introduce the latest glycoprotein analysis technologies and methods. We use diversified analytical technologies to provide clients with accurate and efficient tumor cell line glycoprofiling services. Please contact us if you require specialized glycoprofiling of tumor cell lines.

References

1. Hires, Michal, et al. "Glycan signatures for the identification of cisplatin-resistant testicular cancer cell lines: Specific glycoprofiling of human chorionic gonadotropin (hCG)." Cancer Medicine 11.4 (2022): 968-982.
2. Under Open Access license CC BY 4.0, without modification.

Related Services:

• Glycoprotein Detection
• Glycoprotein Structure Analysis
• Glycoprotein Quantification
• High-throughput Glycan Screening
• Glycosylation Analysis for Virus Glyoprotein
• Antibody Glycoprofiling
• Serum Glycoprofiling
• Plasma Glycoprofiling
• Tumor Tissue Glycoprofiling
• Urine Glycoprofiling
• Quantitative Sialic Acid Analysis
• Quantitative Monosaccharaide Analysis
• Sugar Nucleotide Analysis
• Glycosaminoglycan (GAG) Analysis