3-O-Sulfation Analysis Service

Specialized 3-O-Sulfation Analysis Service at Creative Biolabs

Creative Biolabs has built a mature Polysaccharide Analysis platform, and we provide reliable Heparan Sulfate (HS), 3-O-sulfation analysis, and Fiber Analysis services.

• Sample pretreatment

We perform pre-treatment steps such as desalting and centrifugation on the samples to ensure that the samples have sufficient purity and quality.

• Degradation reaction

We use appropriate enzymes or chemical reagents to degrade the sample into smaller fragments in order to detect 3-O-sulfation modification sites.

• Separation and analysis

We separate different fragments in the sample using techniques such as high-performance liquid chromatography (HPLC) or ion exchange chromatography (IEC). Moreover, mass spectrometry (MS) and nuclear magnetic resonance (NMR) technology are combined to conduct qualitative and quantitative analysis of 3-O-sulfation in different fragments by detecting specific ions or ion fragments. In addition, we set up controlled experiments to verify the existence of 3-O-sulfation through multiple experiments and repeated testing.

At present, we have developed a fast LC-MS/MS method to quantitatively determine low-abundance 3-O-sulfation in HS through ¹³C-labeled 8-mer and 10-mer standards. Since these standards are similar to the 3-O-sulfation structural motif in enzymatically digested HS, this strategy is suitable for analyzing different samples.

Publication

Technology: HPLC and genetic engineering

Journal: Science Advances

IF: 13.116

Published: 2021

Results: HS3ST represents the 3-O-sulfotransferase family. The author used a chemical enzymatic method to synthesize a 3-O-sulfated disaccharide standard (Figure A), and then analyzed the 3-O-sulfated in HS and heparin by HPLC (Figure B). HPLC technology was then combined with genetic engineering of cells lacking HS3ST expression to comprehensively analyze the role of 3-O-sulfation in HS and heparin (Figure C, D, and E). This strategy provided a new basis for sulfation sequence analysis of HS.

Fig.1 Analytical strategies for 3-O-sulfated structures.¹

Advantages

• Professional teams: We have an experienced team of scientific researchers and experts who perform 3-O-sulfation analysis accurately and efficiently.
• Diversified analysis: We use a variety of advanced instruments to provide comprehensive 3-O-sulfation analysis services, including structural analysis, quantitative analysis, and other aspects.
• Efficient service: We actively respond to client needs, and provide rapid 3-O-sulfation analysis services. Moreover, we provide clear and comprehensive data interpretation and reporting.

Application

• Carbohydrate research: Analyzing the position and degree of 3-O-sulfation in the carbohydrate structure helps to study the impact of carbohydrate sulfation modifications (such as heparan sulfate) on cell signaling, cell-cell interactions, and disease development.
• Biological drug development: Analysis of 3-O-sulfation modifications in biological drugs (such as protein drugs) can help evaluate the stability, activity, and immunogenicity of drugs, thereby guiding drug design, optimization, and quality control.
• Structural biology: 3-O-sulfation analysis helps reveal the location and structure of 3-O-sulfation modifications in proteins, sugars, or other biological macromolecules, thereby gaining an in-depth understanding of the functions and interactions of biological molecules.

Creative Biolabs has professional analytical technical teams and a variety of advanced instruments. We have a quality control system and standardized analysis processes to provide clients with accurate and reliable 3-O-sulfation analysis services. Please feel free to contact us if you would like to inquire about specific analysis content.

Reference

1. Karlsson, Richard, et al. "Dissecting structure-function of 3-O-sulfated heparin and engineered heparan sulfates." Science Advances 7.52 (2021): eabl6026.

Related Services:

• Heparan Sulfate Analysis
• Fibre Analysis