Solid Phase-based Glycopeptide Synthesis Service

Professional Solid-Phase Glycopeptide Synthesis Service at Creative Biolabs

There are various linkage types between glycans and designated amino acid residues, such as N-, O-, S-, and C-linkages. Creative Biolabs uses N- and O-glycosylated amino acids as building blocks for standard solid-phase peptide synthesis (SPPS). It is the most common strategic method for the synthesis of glycopeptides, which is used to synthesize small and medium-sized glycopeptide chains containing about 50 amino acids. The synthesis process is fast and simple. We provide different SPPS-based glycopeptide synthesis methods.

• Post-assembly glycosylation
  • Preparation of the solid support: We attach the first amino acid to an appropriate solid support (such as a resin).
  • Assembly of amino acids: We gradually add the desired amino acids at the N-terminus through repeated coupling and deprotection steps until a peptide chain of the desired length is formed.
  • Removal of protecting groups: After each round of amino acid coupling, we need to remove the protecting group so that the N-terminus of the newly added amino acid is in a free state for the next round of reaction.
  • Glycosyl modification: After the peptide chain is constructed, we use appropriate glycation reagents to introduce sugar groups into the peptide chain, and choose appropriate protecting groups to protect the reactive functional groups in the sugar groups.
  • Post-processing: After completing the glycosylation reaction, we isolate the target product through mature purification technology, and use mass spectrometry or other technologies to confirm the structure and purity of the synthesized product.
• Building block approach
  The building block approach is a flexible and efficient method for the synthesis of peptide compounds with specific glycosyl modifications.
  • Preparation of the solid support: We attach the starting amino acids to the solid phase support through appropriate chemical reactions.
  • Prefabrication of glycosyl modules: We design and synthesize appropriate glycosyl modules according to the required glycosyl structure, and use cross-linking agents to connect the glycosyl modules to the peptide chain.
  • Assembly of amino acids: We gradually add different amino acids while adding the appropriate glycosyl modules until the desired peptide chain length is reached.
  • Removal of protecting groups: We push the synthesis reaction forward through the steps of removing protecting groups.
  • Post-processing: After completing the addition of all amino acids, we perform post-reaction processing to ensure the quality and purity of the final synthetic product.

Publication

Technology: Solid phase synthesis

Journal: Organic & Biomolecular Chemistry

IF: 3.89

Published: 2023

Results: The authors developed the high-temperature fast-stirring peptide synthesis (HTFS-PS) strategy, which was combined with low-loading solid supports to efficiently synthesize glycopeptides containing multiple N-glycosylation sites. The results showed that this method was used to synthesize glycosylated peptides, such as N-glycopeptides, O-glycopeptides, and polyphosphorylated peptides.

Fig.1 Installation diagram of HTFS-PS.¹

Advantages

• Professional team: We have experienced and high-quality teams who are familiar with solid-phase synthesis technology and glycopeptide synthesis methods, and they efficiently execute synthesis projects.
• Mature synthesis technology: We use advanced solid-phase synthesis equipment and mature synthesis technology to quickly complete the synthesis of glycopeptides and deliver products in a timely manner.
• High-purity products: We ensure product quality through precise synthesis and strict purification steps, providing high-purity solid-phase glycopeptide products.

Application

• Vaccine development: Glycopeptides play an important role in the field of immunology and serve as vaccine candidates or vaccine adjuvants. Solid-phase synthesis is used to prepare vaccines with specific glycopeptide sequences for activation of the immune system to produce specific antibody responses.
• Glycopeptide analysis: Solid-phase synthesis is used to prepare glycopeptide standards for calibration and quantification of mass spectrometry, chromatography, and other technologies, which is of great significance for studying the structure, composition, and content of glycopeptides.
• Bioengineering: Solid-phase glycopeptide synthesis is also used in the field of bioengineering. By synthesizing glycopeptides of specific sequences, new glycopeptide drugs or functional proteins are designed for applications such as biotherapy, cell engineering, and biosensors.

Glycopeptides are important biomolecules, the synthesis of glycopeptides with specific structures is used in drug development, biological activity research, and other fields. Creative Biolabs provides professional Custom Glycopeptide Synthesis services, including solid-phase glycopeptide synthesis and Liquid phase-based Glycopeptide Synthesis. We design personalized glycopeptide synthesis plans according to client needs and flexibly adjust synthesis strategies to meet different project requirements. Please feel free to contact us if you are interested in our glycopeptide synthesis.

Reference

1. Strauss, Poriah, et al. "Accelerated solid-phase synthesis of glycopeptides containing multiple N-glycosylated sites." Organic & Biomolecular Chemistry 21.8 (2023): 1674-1679.

Related Services:

• Liquid phase-based Glycopeptide Synthesis