Plant Hormone Compound Analysis Service

Precision Plant Hormone Analysis Solutions at Creative Biolabs

Plant hormones function as biochemical conductors, coordinating growth patterns and stress responses across botanical systems. Unlike animal counterparts produced in discrete glands, these signaling molecules are synthesized in various tissues with both localized and systemic effects. Accurate understanding of plant hormone levels is critical to optimizing crop yields, enhancing stress resistance, and promoting advances in plant biotechnology. Creative Biolabs provides comprehensive Plant Chemistry Profiling services, including Plant Pigment, Element, Vitamin, hormone, etc., which empower users to delve deeper into plant biology.

• Plant Hormone Compound Analysis Service

Focusing on minute but potent phytohormones like ethylene and jasmonates, our liquid chromatography-mass spectrometry (LC-MS) platforms detect compounds active at picomolar concentrations. The service accommodates diverse analytes from classical auxins to modern brassinosteroids, capturing their dynamic interplay within plant systems.

Table 1 Plant hormone compounds detection list.

• Gibberellin Compound Analysis Service

Gibberellin compounds are a class of plant hormones. It is derived from tetracyclic diterpene skeletons, including more than 130 structural variants (such as GA3 and GA4). They promote stem elongation, seed germination, and fruit development by activating GID1 receptors to degrade DELLA proteins. Due to the significant functional differences of different gibberellin isomers and the complex metabolic dynamics in plants, we use specific LC-MS/MS analysis to detect various gibberellins.

Table 2 Gibberellin compounds detection list.

• Brassinosteroid Compound Analysis Service

Brassinolide compounds are plant hormones with steroids as the skeleton, represented by brassinolide, which are multifunctional regulatory hormones with both growth promotion and stress resistance. Its content is extremely low, and slight structural differences can significantly affect its activity. We use highly sensitive LC-MS/MS or bromination-derived gas phase mass spectrometry (GC-MS) for accurate quantification. Individual analysis of this type of compound optimizes stress-resistance breeding strategies and provides key metabolic data for the development of drought-resistant and salt-alkali-resistant crops.

Table 3 Brassinolide compounds detection list.

Sample Requirements and Analysis Process

Creative Biolabs can analyze a wide range of plant samples. The analysis process typically involves sample preparation, chromatographic separation, MS detection, and identification analysis.

Creative Biolabs specializes in delivering precise phytohormone analysis solutions tailored to both academic and industrial requirements. Our methodology harnesses state-of-the-art analytical platforms to support groundbreaking discoveries in plant science. For protocol customization or project consultations, our technical team remains available for immediate discussion. Please contact us to customize your plant hormone analysis service.

Published Data

The authors developed an LC-MS/MS assay for accurate, reliable, and comparable measurement of key phytohormone levels in Arabidopsis thaliana. The experimental design quantified six critical regulators:

• Auxin (IAA)
• Stress hormones ABA and JA
• Signaling molecules SA and OPDA
• Jasmonate conjugate JA-Ile

The mass spectral analysis employed ion trap technology to achieve cross-laboratory reproducibility - a longstanding challenge in comparative phytohormone studies. This standardized protocol enables consistent data generation across varied experimental setups.

Fig.1 Selected reaction monitoring (SRM) chromatograms of phytohormones in samples.¹

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Reference

1. Almeida Trapp, Marília, et al. "Validated method for phytohormone quantification in plants." Frontiers in Plant Science 5 (2014): 417. Distributed under Open Access license CC BY 4.0, without modification.

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