Precise Quantification of Amino Sugars: Enabling Advances in Glycobiology
Amino sugars are critical components of glycoproteins, glycolipids, and microbial polysaccharides and play pivotal roles in biological processes ranging from cellular communication to pathogen-host interactions. Accurately quantifying these molecules is essential for advancing research in drug development, disease diagnostics, and biomanufacturing. Creative Biolabs is a leader in Carbohydrate Analysis services, providing cutting-edge amino sugar quantitative analysis services designed to address the evolving demands of academia and industry. The amino sugar quantitative analysis service at Creative Biolabs caters to a broad spectrum of sample types, ensuring comprehensive analysis across different research and industrial domains.
Rigorous Analysis Process
-
Sample preparation: We provide expert guidance on sample preparation, including enzymatic hydrolysis, chemical derivatization, and purification, to ensure optimal analyte recovery and minimize matrix interference.
-
Sample detection: Our professional scientists develop and validate customized analysis methods applied to the specific requirements of each project, adhering to rigorous quality control standards.
-
Data analysis: We deliver comprehensive data reports, including quantitative results, chromatograms, and spectral data, accompanied by expert interpretation.
-
Customized solutions: We provide flexible service options to accommodate diverse research needs, including high-throughput analysis, specialized sample handling, and custom data reporting.
Advanced Analysis Techniques
-
Dual-platform: We employ a dual-platform approach combining high-performance liquid chromatography (HPLC) and tandem mass spectrometry (LC-MS/MS) to achieve unparalleled sensitivity in amino sugar detection. The service specializes in quantifying key molecules such as glucosamine, galactosamine, etc., even at trace concentrations (detection limits as low as 0.1 ng/mL). Proprietary chromatographic columns and optimized mobile phases ensure baseline separation of structurally similar analytes, minimizing matrix interference from complex biological samples.
-
High resolution: High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is a method based on anion exchange chromatography separation combined with electrochemical detection, which is particularly well-suited for analyzing complex mixtures of amino sugars. This technique enables the accurate determination of individual amino sugar concentrations, even in samples with low abundance.
Creative Biolabs provides clients with high-quality, reliable, and timely amino sugar quantitative analysis services. In addition, we also provide various carbohydrate analysis services, including Monosaccharide, Oligosaccharide, and Polysaccharide. Our team of experienced scientists, coupled with our cutting-edge technology, ensures accurate and reproducible results, accelerating your research and development efforts. Please contact us to inquire about more details if you are very interested in our mature analysis services.
Published Data
This study investigated changes in microbial communities under long-term exposure to global change factors (increasing CO2 concentrations, warming, and nitrogen deposition). The ratio of total amino sugars to total soil carbon has been utilized to assess the relative impact of microbial communities on the turnover and storage of soil carbon. Lipids and amino sugars were used as biomarkers. Lipid analysis provided insight into living microbial biomass, while amino sugar analysis was used to quantify microbial residues. The figure below illustrates the absolute abundance of total amino sugars as well as individual amino sugars under both single and combined global change treatments. The study showed that total amino sugar concentrations (including glucosamine, galactosamine, and mannosamine) decreased under all global change treatments. In contrast, the bacterial marker muramic acid increased. Temperature and nitrogen deposition were associated with lower levels of total amino sugars and higher muramic acid.
Fig.1 Absolute abundance of total and individual amino sugars under global change treatments.1
FAQs
Q1: What specific amino sugars can your service quantify?
A1: Our service is versatile and quantifies a wide range of amino sugars, including glucosamine, galactosamine, N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), muramic acid, and sialic acids. We also develop methods for quantifying less common amino sugars according to your specific needs.
Q2: What is the detection limit and dynamic range of your quantification method?
A2: Our detection limits typically range from picomolar to nanomolar levels, depending on the specific amino sugar and matrix. The dynamic range is broad, often spanning several orders of magnitude, ensuring accurate quantification across various sample concentrations. We provide detailed validation data for each method, including the limit of detection and the limit of quantification.
Q3: Can you develop a customized method for a novel amino sugar or a complex sample matrix?
A3: Absolutely. We have a team of experienced analytical chemists who specialize in method development and optimization. We tailor our methods to meet your specific requirements, including developing new derivatization procedures, chromatographic separations, and mass spectrometry methods for unique amino sugars or challenging matrices.
Customer Review
Professional Statistical Analysis
"The statistical analysis performed by Creative Biolabs was thorough and insightful. It helped me identify significant differences between my sample groups and draw meaningful conclusions."
Efficient Delivery of Reports
"The turnaround time exceeded expectations, and the quality of the data was exceptional. I was impressed by their efficiency and precision."
Reference
-
Liang, Chao, Jessica LM Gutknecht, and Teri C. Balser. "Microbial lipid and amino sugar responses to long-term simulated global environmental changes in a California annual grassland." Frontiers in Microbiology 6 (2015): 385. Distributed under Open Access license CC BY 4.0, without modification.
Related Services
For Research Use Only.
Contact Us
Follow us on
