Introduction of Arachidonic Acid
Arachidonic acid (AA), a key omega-6 fatty acid, is a precursor to many potent lipid mediators known as eicosanoids, including prostaglandins, leukotrienes, and thromboxanes. These molecules play pivotal roles in regulating fundamental biological processes such as inflammation, immune response, pain, and cardiovascular function. Dysregulation of AA metabolism is implicated in numerous pathologies, from cardiovascular diseases and cancer to neurological disorders and inflammatory conditions like benign prostatic hyperplasia. Accurate quantification of AA and its metabolites is therefore critical for understanding disease mechanisms, identifying therapeutic targets, and discovering novel biomarkers. Creative Biolabs leverages state-of-the-art ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technology to provide precise and comprehensive analysis, supporting robust scientific inquiry.
AA Quantitative Analysis Service at Creative Biolabs: Precision in Lipidomics, Powering Your Research
At Creative Biolabs, our AA analysis service provides precise and reliable quantification of AA and its diverse metabolites, known as eicosanoids, in various biological matrices. This service delivers critical insights into inflammatory processes, disease progression, and therapeutic responses, enabling researchers to identify potential biomarkers, understand complex signaling pathways, and validate drug targets. Our deliverables include highly accurate concentration data, comprehensive analytical reports, and expert interpretation to guide your project's next steps.
Creative Biolabs provides flexible options for AA quantitative analysis to meet diverse research needs:
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Targeted AA analysis: Focused quantification of specific, pre-defined panels of AA metabolites.
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Untargeted lipidomics screening: We can also integrate with broader untargeted lipidomics approaches to provide a more holistic view of lipid metabolism, identifying novel or unexpected lipid species.
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Custom assay development: For unique research requirements or novel analytes, we provide custom method development and validation services to quantify specific AA metabolites not covered in our standard panels.
As an important polyunsaturated fatty acid, AA and its metabolites have a variety of biological effects on the body. These compounds are formed through different enzymatic reactions and participate in the regulation of inflammation, immunity, and other physiological functions. We provide a diverse range of analysis services for AA and its metabolites.
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Prostaglandins
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Prostaglandin A2, prostaglandin D2, prostaglandin E2, prostaglandin J2
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These prostaglandins are converted from AA through the cyclooxygenase (COX) pathway. They play an important role in regulating blood pressure, inflammatory response, pain, and other physiological processes.
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Hemoglobin
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Hemoglobin B2
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This is a compound generated from AA, which participates in the regulation of inflammation and immune response and is an important biologically active lipid.
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Leukotrienes
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Leukotriene B4, 6-trans-leukotriene B4
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Leukotrienes are synthesized from AA through the lipoxygenase (LOX) pathway. They play an important role in immune response and inflammatory processes, especially in attracting white blood cells.
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Hydroxydiacids
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5-Hydroxyhexanedioatetraenoic acid, 12-Hydroxyhexanedioatetraenoic acid, 15-Hydroxyhexanedioatetraenoic acid
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These compounds are hydroxy derivatives of AA, involved in lipid metabolism, and may play a role in cell signaling.
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Epoxides
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5-Oxo-6,8,11,14-eicosatetraenoic acid, 8,9-epoxyeicosatrienoic acid, 11,12-epoxy-5,8,14-eicosatrienoic acid, 14,15-epoxy-5,8,11-eicosatrienoic acid
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These epoxides are metabolites of AA and its analogs, usually with strong biological activity, and are involved in a variety of biological processes, including inflammation, cell proliferation, and apoptosis.
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Creative Biolabs is your trusted partner for advanced AA quantitative analysis, delivering the precise, actionable data you need to drive your scientific discoveries forward. Our cutting-edge technology, rigorous methodology, and dedicated team of experts ensure unparalleled accuracy and support for your most challenging research questions. In addition, we also provide analysis for other compounds related to Metabolic Research, such as Carotenoid, Anthocyanin, Flavonoid, and Trimethylamine N-oxide (TMAO) quantitative analysis services. Please contact us today to discuss your project, learn more about our capabilities, and receive a customized quote. We are here to answer your questions and help you achieve your research goals.
Related Services
To further support your comprehensive research needs, Creative Biolabs provides a suite of complementary services that can be integrated with our AA quantitative analysis service:
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Targeted Lipidomics: Explore other crucial lipid classes and their metabolites beyond AAs.
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Untargeted Metabolomics: Gain a holistic view of your sample's metabolome, identifying a broad range of small molecules.
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Proteomics Services: Investigate protein expression and post-translational modifications to understand the enzymatic machinery driving lipid metabolism.
Published Data
This research details the development and validation of a sophisticated UHPLC-MS/MS analysis technique, designed to precisely measure AA and its various metabolic byproducts. These specific metabolites are generated through the cyclooxygenase (COX) and lipoxygenase (LOX) pathways within biological tissues. The study's primary application of this method was to investigate changes in these compounds in rat models of benign prostatic hyperplasia (BPH). The findings revealed a significant elevation in the levels of eleven distinct biomarkers across multiple tissues, including the prostate, seminal vesicles, bladder, liver, and kidneys, in the BPH-affected rats when compared to a control group. This suggests that BPH intensifies the production of arachidonic acid and its eicosanoid derivatives, likely by promoting the activity of COX and LOX enzymes.
Fig.1 Detection results of AA and its metabolites in different tissues.1
FAQs
Q1: What types of samples can I submit for AAcid quantitative analysis?
A1: We accept a wide range of biological samples, including various tissue types (e.g., prostate, liver, kidney, lung), plasma, serum, urine, cell lysates, and cell culture supernatants. Proper sample collection and storage are crucial for accurate results.
Q2: How sensitive is Creative Biolabs' method for detecting AA metabolites?
A2: Our UHPLC-MS/MS platform offers exceptional sensitivity, allowing for the detection and quantification of AA metabolites at very low physiological concentrations. This high sensitivity is critical for identifying subtle changes in lipid mediator profiles, even in limited sample volumes.
Q3: How does Creative Biolabs' service compare to other analytical approaches like ELISA kits?
A3: Our UHPLC-MS/MS service provides superior specificity, sensitivity, and multiplexing capabilities compared to traditional ELISA kits. While ELISAs are useful for single-analyte screening, our method allows for the simultaneous quantification of multiple AA metabolites with higher accuracy and less cross-reactivity, providing a more comprehensive and reliable lipidomics profile.
Customer Review
Exceptional Precision
"Using Creative Biolabs's AA quantitative analysis service in our research has significantly improved the precision of our lipid mediator quantification. The detailed reports allowed us to confidently identify subtle changes in eicosanoid profiles, which were crucial for our neuroinflammation study." - Prof. G. Mil***r
Reliable Biomarker Discovery
"We struggled with the consistent detection of certain eicosanoids using our in-house methods. Creative Biolabs provided highly reliable and reproducible data, which directly led to the discovery of a promising new biomarker for metabolic syndrome. The precautions they took to ensure sample integrity were also very reassuring." - Dr. H. Dav***d
Reference
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Bian, Qiaoxia, et al. "Quantification of arachidonic acid and its metabolites in rat tissues by UHPLC-MS/MS: application for the identification of potential biomarkers of benign prostatic hyperplasia." PLoS One 11.11 (2016): e0166777. DOI: 10.1371/journal.pone.0166777. Distributed under Open Access license CC BY 4.0, without modification.
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