Carbohydrate Chain Research

Why Carbohydrate Chains?

Carbohydrate chains are important biomolecules composed of monosaccharides, oligosaccharides, and polysaccharides. They contribute significantly to glycobiology by glycosylating proteins and lipids, which has a direct impact on cell communication, immunological recognition, and disease mechanisms. These chains facilitate cell communication, guide immune responses, and potentially identify cancer cells for targeted therapy. They have an impact on everything in biological processes, including tissue formation and metabolic regulation. At Creative Biolabs, we investigate how carbohydrate chains influence vaccination efficacy, stem cell therapies, and disorders such as neurological diseases or infections. Understanding their structure and function enables us to develop novel diagnostic tools and treatments for complex health issues.

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Deeper Understanding of Carbohydrate chains

Glycan structure basis. (Creative Biolabs Authorized)

Carbohydrate Chain Basis

Sugar-based structures (mono-/oligo-/polysaccharides) that shape biomolecule functions, from cell identity to therapeutic targeting.

Monosaccharide Overview Oligosaccharide Overview Polysaccharides Overview

Glycan in vaccine development. (Creative Biolabs Authorized)

Carbohydrate Chain in Glycobiology

Learn how glycosylation drives cell communication, disease pathways, and vaccine design through sugar-protein interactions.

In Disease In Cancer In Vaccine

Glycan in biological process. (Creative Biolabs Authorized)

Carbohydrate Chain in Biological Processes

See sugars steering immunity, tissue repair, and infections – keys to innovative diagnostics and treatments.

In Immunity In Cell Signaling In Stem Cells In Tissue Engineering In Brain Development and Neurodegenerative Diseases In Aging In Microbial Infections In Metabolic Processes In Cellular Stress In Lipid Metabolism and Membrane Fluidity In Bone Marrow In Blood Cells In Liver Function In Endocrine Function In Kidney Function In Eye Health In Reproductive In Heart Disease In Gastrointestinal Health In Inflammation

Advanced Technologies for Glycan Research

Classification	Technology	Description
Liquid Chromatography Techniques (For Glycan Separation and Purification)	HPLC	Separates glycans based on polarity and molecular interactions.
	HPAEC-PAD	Detects and quantifies glycans based on charge differences.
	RP-HPLC	Separates glycopeptides and glycans based on hydrophobicity.
	UHPLC/FLD/Q-TOF	High-resolution glycan and glycopeptide profiling.
Mass Spectrometry Techniques (For Glycan and Glycopeptide Identification)	MS	Identifies glycan structures with high sensitivity.
	LC-ESI-MS	Combines chromatography and MS for glycoprotein characterization.
	MALDI-TOF MS	Rapid glycan and glycopeptide profiling with minimal sample prep.
	GC-MS	Analyzes glycan derivatization products with high resolution.
	Imaging MS	Spatial distribution mapping of glycans on tissue samples.
Other Spectroscopy Techniques (For Functional Group and Conformational Analysis)	FTIR	Detects glycan functional groups via vibrational spectroscopy.
	NMR	Determines glycan conformations and structural details.
	TLC	Simple glycan separation and qualitative analysis.

Classification	Technology	Description
Glycobiology Microarray (For High-Throughput Glycan-Protein Interaction Screening)	Lectin Microarray	High-throughput detection of glycan-binding interactions.
	100 Glycan Microarray	Screening platform for diverse glycan structures.
	100 N-Glycan Microarray	N-glycan-specific profiling and interaction studies.
	Sialic Acid Microarray	Specific to sialylated glycans in functional assays.
	Glycosphingolipid Microarray	Focuses on glycosphingolipid-glycan interactions.
	Glycosaminoglycan Microarray	Specialized for glycosaminoglycan-binding proteins.
	Human Milk Oligosaccharide Microarray	Targets human milk-derived glycans.
	Glycoprotein Microarray	Broad profiling of glycoproteins and interactions.
	Glycopeptide Microarray	Focused on glycopeptide-based interactions.
Biosensing and Cellular-Level Analysis (For Real-Time Glycosylation Monitoring)	SPRi	Real-time analysis of glycan-protein binding kinetics.
	Flow Cytometry	Cell surface glycan profiling via fluorescence detection.

Services You May Be Interested in

At Creative Biolabs, we are committed to delivering high-quality and reliable carbohydrate research services to accelerate your scientific discoveries.

Glycan Synthesis

We provide custom synthesis of various glycans, including monosaccharides, oligosaccharides, polysaccharides, and carbohydrate antigens to support your research needs.

Fig.1 Glycan synthesis strategies.^1,7

Carbohydrate Analysis

Our carbohydrate analysis services ensure accurate structural characterization and quantification of monosaccharides, oligosaccharides, and polysaccharides.

Fig.2 Total cell wall Analytical methods.

Fig.2 Analytical methods for total cell wall analysis.^2,7

Glycan Modification and Labeling

We offer modification and labeling services to enhance the functionality and detection of glycans in biological systems.

Fig.3 Workflow from sample preparation to O-glycan analysis.^3,7

Glycoengineering

Our glycoengineering solutions help optimize glycoproteins, antibodies, and cell lines for therapeutic and biotechnological applications.

Fig.4 The Fc N-glycan and common biantennary complex-type N-glycans structures.

Fig.4 Structure of the Fc N-glycan and common biantennary complex-type N-glycans.^4,7

Related Glycan Products

Carbohydrate Chains in Action

The research identifies how carbohydrate chains play a critical function in disease development with a specific focus on rheumatoid arthritis (RA). Researchers identified distinct glycosylation modifications in serum IgG through high-throughput lectin microarray analysis which could be associated with RA. Distinct glycosylation patterns in RA patients influence both immune responses and inflammation processes. Scientists now understand RA’s fundamental processes better through these results while they also create possibilities for new diagnostic methods and therapeutic approaches. Research into carbohydrate chain modifications holds promise for enhancing early detection and developing precise treatments for RA and RA-ILD.

Fig.5 Lectin microarray. Fig.5 The process of lectin microarray. ^5,7

Fig.6 Altered glycosylation in serum IgG Fig.6 Modified glycosylation in serum IgG.^5,7

Mannan, a polysaccharide extracted from yeast cell walls, has become an efficient vaccine carrier because of its distinctive carbohydrate chains. These carbohydrate chains bind to mannose receptors on immune cells which initiates an immune response cascade. Researchers conjugated mannan with MUC1 tumor antigen using oxidative and reductive techniques in one well-documented example. The oxidized mannan-MUC1 fusion protein generated a strong Th1 immune response which activated cytotoxic T cells and gave substantial protection in mice with tumors. Research demonstrates carbohydrate chains play a vital role in boosting antigenicity and guiding immune responses which shows mannan's promise for vaccine development.

Fig.7 Mannan and oxidized & non-oxidized mannan conjugate structures. Fig.7 The structure of mannan and oxidized & non-oxidized mannan conjugate.^6,7

What Our Customers Say

"Creative Biolabs provided outstanding custom glycan synthesis services for our study on glycoprotein interactions. The glycans were highly pure and structurally accurate, allowing us to achieve reliable experimental results. Their technical support was also incredibly helpful. I highly recommend their custom glycan synthesis services for anyone working in glycoscience!"

– Dr. Emily Carter, Glycobiology Researcher

"We needed detailed monosaccharide and oligosaccharide analysis for our drug development project, and Creative Biolabs delivered precise and timely results. Their expertise in carbohydrate analysis services helped us better understand glycan structures and improve our formulations. Their efficient communication and project updates made the process smooth and stress-free."

– Dr. James Liu, Pharmaceutical Scientist

"Our research on autoimmune diseases required specialized glycan modifications for detection assays. The glycan modification and labeling services at Creative Biolabs were exactly what we needed. The team was knowledgeable, and their biotinylation and fluorescence labeling services greatly enhanced our study. The fast turnaround time and data consistency were particularly impressive."

– Prof. Anna Rodriguez, Immunology Researcher

FAQs

Can you synthesize custom glycans for my research?

Yes, we specialize in custom glycan synthesis services, including monosaccharides, oligosaccharides, and polysaccharides tailored to your research needs. Our team ensures high purity and structural accuracy to support drug development, vaccine research, and glycomics studies.

How do you analyze carbohydrate structures in complex biological samples?

We employ advanced analytical techniques such as mass spectrometry (MS), high-performance liquid chromatography (HPLC), and lectin-based assays to provide detailed carbohydrate profiling. Our carbohydrate analysis services help researchers identify glycan structures and modifications in various biological samples.

How can glycoengineering improve therapeutic proteins and antibodies?

Glycoengineering enhances the efficacy, stability, and immunogenicity of biopharmaceuticals. We offer therapeutic protein glycoengineering services and therapeutic antibody glycoengineering services to optimize glycosylation patterns for better therapeutic outcomes.

References

Hamilton, Brian S., et al. "A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors." Scientific reports 7.1 (2017): 15907. https://doi.org/10.1038/s41598-017-15891-8
Tingley, Jeffrey P., et al. "Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues." Biotechnology for Biofuels 14 (2021): 1-19. https://doi.org/10.1186/s13068-021-01888-z
Safferthal, Marc, et al. "Labeling of mucin-type O-glycans for quantification using liquid chromatography and fluorescence detection." ACS Measurement Science Au 4.2 (2024): 223-230. https://doi.org/10.1021/acsmeasuresciau.3c00071
Li, Shasha, et al. "Glycoengineering of therapeutic antibodies with small molecule inhibitors." Antibodies 10.4 (2021): 44. https://doi.org/10.3390/antib10040044
Deng, Xiaoyue, et al. "Changes of serum IgG glycosylation patterns in rheumatoid arthritis." Clinical proteomics 20.1 (2023): 7. https://doi.org/10.1186/s12014-023-09395-z
Lang, Shuyao, and Xuefei Huang. "Carbohydrate conjugates in vaccine developments." Frontiers in Chemistry 8 (2020): 284. https://doi.org/10.3389/fchem.2020.00284
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