0
Inquiry Basket

There is no product in the shopping cart, buy it!

Quantitative Human DPAGT1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX807)

Datasheet
SizeQtyAdd To Basket
1×48 T
1×96 T

Product Description The quantitative human DPAGT1 (glycosylated) sandwich ELISA kit is designed to detect human dolichyl phosphate-N-acetylglucosaminephosphotransferase 1 (DPAGT1) levels. DPAGT1 is an enzyme that is located in the endoplasmic reticulum membrane. DPAGT1 catalyzes the first step in the glycosylation pathway. This enzyme is essential for the synthesis of N-glycans, which are crucial for protein folding and functions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of DPAGT1 in the sample.
Target DPAGT1
N-Glycosylation Site 146
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.093 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Dolichyl phosphate-N-acetylglucosaminephosphotransferase 1
Alternate Names DPAGT1; Dolichyl phosphate-N-acetylglucosaminephosphotransferase 1; DGPT; DPAGT; G1PT; UAGT; GlcNAc-1-P transferase
Uniprot No. Q9H3H5
Application The quantitative human DPAGT1 (glycosylated) sandwich ELISA kit provides a means to quantitatively measure DPAGT1 levels in biological samples. It is valuable for studies examining protein glycosylation. This kit has applications in research focused on congenital disorders of glycosylation. It is also relevant to studies investigating the role of glycosylation in various cellular processes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
Online Inquiry
Creative Biolabs-Glycoprotein Follow us on
  • ()
    ()
    ()
ISO 9001 Certified - Creative Biolabs Quality Management System.
Close
Thanksgiving
Thanksgiving