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Quantitative Human EIF2αK3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX860)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EIF2αK3 (glycosylated) sandwich ELISA kit is designed to detect human eukaryotic translation initiation factor 2α kinase 3 (EIF2αK3) levels. EIF2AK3 is a protein kinase that is located in the endoplasmic reticulum. EIF2AK3 is activated by endoplasmic reticulum stress. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of EIF2αK3 in the sample.
Target EIF2αK3
N-Glycosylation Site 258
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.091 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Eukaryotic translation initiation factor 2α kinase 3
Alternate Names EIF2αK3; Eukaryotic translation initiation factor 2α kinase 3; PERK; PRKR-like endoplasmic reticulum kinase
Uniprot No. Q9NZJ5
Application The quantitative human EIF2αK3 (glycosylated) sandwich ELISA kit is used to determine EIF2AK3 levels in biological samples. This kit is applicable in studies examining endoplasmic reticulum stress and cellular stress. It is also relevant to studies investigating the role of EIF2AK3 in regulating protein synthesis during stress and its involvement in various diseases, including neurodegenerative disorders and diabetes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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